Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,322,699	+A	coding (746/759 nt)	phnK ←	carbon‑phosphorus lyase complex subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,322,695	1	.	A	100.0%	53.8 / NA	18	L250F (TTG→TTT)	phnK	carbon‑phosphorus lyase complex subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (18/0);  total (18/0)

CATCCGGCACCGGCTCAATTCTGC‑AAAACCGATGACACCAGCAGCTGTGTATACG  >  W3110S.gb/4322672‑4322726                         |                                cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:2545059/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:966926/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:542313/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:3127981/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:3117195/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:3030232/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:3019186/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:2880953/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:2810139/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:1270749/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:2389086/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:2204880/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:2071742/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:2027585/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:2005312/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:1973348/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:1886030/1‑56 (MQ=255) cATCCGGCACCGGCTCAATTCTGCAAAAACCGATGACACCAGCAGCTGTGTATACg  >  1:1334280/1‑56 (MQ=255)                         |                                CATCCGGCACCGGCTCAATTCTGC‑AAAACCGATGACACCAGCAGCTGTGTATACG  >  W3110S.gb/4322672‑4322726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑