Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,357,180	(A)8→9	intergenic (+143/‑84)	yjdJ → / → yjdK	predicted acyltransferase with acyl‑CoA N‑acyltransferase domain/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,357,172	1	.	A	100.0%	32.7 / NA	12	intergenic (+135/‑92)	yjdJ/yjdK	predicted acyltransferase with acyl‑CoA N‑acyltransferase domain/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (7/5);  total (7/5)

CACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑AAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGC  >  W3110S.gb/4357116‑4357227                                                          |                                                        cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc                                                          <  1:3083471/57‑1 (MQ=255) cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc                                                          <  1:30388/57‑1 (MQ=255) cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc                                                          <  1:1382433/57‑1 (MQ=255) cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc                                                          <  1:1415061/57‑1 (MQ=255) cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc                                                          <  1:1746540/57‑1 (MQ=255) cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc                                                          <  1:3019355/57‑1 (MQ=255) cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc                                                          <  1:2024286/57‑1 (MQ=255) cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑aaaaa                                                    <  1:1686952/62‑1 (MQ=255) cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑aaaaa                                                    <  1:1791503/62‑1 (MQ=255) cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑aaaaa                                                    <  1:196615/62‑1 (MQ=255) cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑aaaaa                                                    <  1:773753/62‑1 (MQ=255)                  gagCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAAGAATATCTCCta                                     <  1:1261443/61‑1 (MQ=255)                  gagCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAAGAATATCTCCta                                     <  1:2107883/61‑1 (MQ=255)                        gTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAAGAATATCTCCTATATgag                               <  1:1758915/61‑1 (MQ=255)                               tGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAAGAATATCTCCTATATGAGAatcatc                        <  1:1032583/61‑1 (MQ=255)                                              ccTTTACTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAg        >  1:2087335/1‑62 (MQ=255)                                              ccTTTACTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAg        >  1:716182/1‑62 (MQ=255)                                                    cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGtttt    <  1:1179099/60‑1 (MQ=255)                                                    cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTg   >  1:2624726/1‑61 (MQ=255)                                                    cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTg   >  1:1752336/1‑61 (MQ=255)                                                    cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGc  >  1:863887/1‑62 (MQ=255)                                                    cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGc  >  1:1101056/1‑62 (MQ=255)                                                    cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAAAAAGTTTTGc  >  1:259575/1‑62 (MQ=255)                                                          |                                                        CACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑AAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGC  >  W3110S.gb/4357116‑4357227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑