| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 698,061 | (GCAC)2→3 | coding (1539/1665 nt) | asnB ← | asparagine synthetase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 698,053 | 1 | . | G | 81.8% | 27.5 / 1.1 | 11 | coding (1547/1665 nt) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (9/0); total (9/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | W3110S.gb | 698,053 | 2 | . | C | 81.8% | 25.1 / 1.4 | 11 | coding (1547/1665 nt) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (9/0); total (9/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 | |||||||||||
| * | W3110S.gb | 698,053 | 3 | . | A | 81.8% | 22.0 / 1.9 | 11 | coding (1547/1665 nt) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (9/0); total (9/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
| * | W3110S.gb | 698,053 | 4 | . | C | 81.8% | 27.7 / 1.4 | 11 | coding (1547/1665 nt) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (9/0); total (9/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
TCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCG‑‑‑‑GCACGCACTCAGCGGCGCTCGGA > W3110S.gb/698015‑698076 |||| tCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCG‑‑‑‑GCACGCACTCAGCGGCGCTCgg < 1:830307/61‑1 (MQ=255)tCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCG‑‑‑‑GCACGCACTCAGCGGCGCTCgg < 1:86304/61‑1 (MQ=255) tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCgg > 1:1386072/1‑61 (MQ=255) tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:1091759/1‑62 (MQ=255) tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:1310596/1‑62 (MQ=255) tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:1327613/1‑62 (MQ=255) tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:2468288/1‑62 (MQ=255) tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:2726761/1‑62 (MQ=255) tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:3111558/1‑62 (MQ=255) tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:612543/1‑62 (MQ=255) tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:85570/1‑62 (MQ=255) |||| TCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCG‑‑‑‑GCACGCACTCAGCGGCGCTCGGA > W3110S.gb/698015‑698076 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |