| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 770,570 | +G | coding (2171/2634 nt) | mngB → | alpha‑mannosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 770,567 | 1 | . | G | 88.9% | 23.3 / ‑2.6 | 9 | coding (2168/2634 nt) | mngB | alpha‑mannosidase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (0/8); total (0/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
GGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTC‑GGGAATTAAAATGCCAGTCCCGG > W3110S.gb/770521‑770590 | ggCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTC‑GGGAATTAAAATGcc < 1:1619420/62‑1 (MQ=255) agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:1090109/54‑1 (MQ=255) agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:1091931/54‑1 (MQ=255) agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:1144827/54‑1 (MQ=255) agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:1987941/54‑1 (MQ=255) agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:2420853/54‑1 (MQ=255) agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:2432382/54‑1 (MQ=255) agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:2797325/54‑1 (MQ=255) agaTCTGCTTTTAAGGCCTGGGCGGCCTTCGGGGAATTAAAATGCCAGTCCCgg < 1:668066/54‑1 (MQ=255) | GGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTC‑GGGAATTAAAATGCCAGTCCCGG > W3110S.gb/770521‑770590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |