Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	977,292	+G	coding (545/4461 nt)	mukB →	fused chromosome partitioning proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	977,289	1	.	G	100.0%	30.5 / NA	10	L181R (CTG→CGG)	mukB	fused chromosome partitioning proteins
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (10/0);  total (10/0)

ACCACTCGCTGATGTTCGATCT‑GGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAC  >  W3110S.gb/977268‑977326                       |                                      aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc  >  1:1109382/1‑60 (MQ=255) aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc  >  1:1301731/1‑60 (MQ=255) aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc  >  1:1758010/1‑60 (MQ=255) aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc  >  1:2541628/1‑60 (MQ=255) aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc  >  1:2569239/1‑60 (MQ=255) aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc  >  1:2596627/1‑60 (MQ=255) aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc  >  1:2651137/1‑60 (MQ=255) aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc  >  1:2661857/1‑60 (MQ=255) aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc  >  1:944375/1‑60 (MQ=255) aCCACTCGCTGATGTTCGATCTGGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc  >  1:977985/1‑60 (MQ=255)                       |                                      ACCACTCGCTGATGTTCGATCT‑GGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAC  >  W3110S.gb/977268‑977326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑