| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,141,889 | (A)6→7 | coding (280/954 nt) | flgL → | flagellar hook‑filament junction protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,141,883 | 1 | . | A | 77.8% | 16.1 / 2.1 | 9 | coding (274/954 nt) | flgL | flagellar hook‑filament junction protein |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (7/0); total (9/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGG‑AAAAAATTGTCTACGCCAGCAATGGCA > W3110S.gb/1141846‑1141910 | aCTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAAATTGTCTACGCCAGCAAt > 1:1152713/1‑62 (MQ=255)aCTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAAATTGTCTACGCCAGCAAt > 1:1851578/1‑62 (MQ=255)aCTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAAATTGTCTACGCCAGCAAt > 1:2017011/1‑62 (MQ=255)aCTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAAATTGTCTACGCCAGCAAt > 1:2855836/1‑62 (MQ=255)aCTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAAATTGTCTACGCCAGCAAt > 1:3074948/1‑62 (MQ=255)aCTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAAATTGTCTACGCCAGCAAt > 1:971579/1‑62 (MQ=255)aCTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGGAAAAAAATTGTCTACGCCAGCAAt > 1:974159/1‑62 (MQ=255) tAGCCAGGTCACCACTGCTATCCAGAATGCTCAGG‑AAAAAATTGTCTACGCCAGCAATGGCa > 1:1385982/1‑62 (MQ=255) tAGCCAGGTCACCACTGCTATCCAGAATGCTCAGG‑AAAAAATTGTCTACGCCAGCAATGGCa > 1:1578546/1‑62 (MQ=255) | ACTTAGCCAGGTCACCACTGCTATCCAGAATGCTCAGG‑AAAAAATTGTCTACGCCAGCAATGGCA > W3110S.gb/1141846‑1141910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |