Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,593,051	(A)6→7	coding (840/1323 nt)	hipA ←	regulator with hipB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,593,045	1	.	A	100.0%	26.9 / NA	10	L282F (TTG→TTT)	hipA	regulator with hipB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (10/0);  total (10/0)

ACCTGGAATTTCATAAAATCATAGCGATCTTTCAGCGCCTCGCTGGACCCCATC‑AAAAAAGCCATGATCCGCGCGATGCCTGGGCCTCCATCTGATT  >  W3110S.gb/1592992‑1593088                                                       |                                            aCCTGGAATTTCATAAAATCATAGCGATCTTTCAGCGCCTCGCTGGACCCCATC‑aaaaaaag                                     <  1:1051493/62‑3 (MQ=255) aCCTGGAATTTCATAAAATCATAGCGATCTTTCAGCGCCTCGCTGGACCCCATC‑aaaaaaag                                     <  1:552980/62‑3 (MQ=255) aCCTGGAATTTCATAAAATCATAGCGATCTTTCAGCGCCTCGCTGGACCCCATC‑aaaaaaag                                     <  1:260225/62‑3 (MQ=255) aCCTGGAATTTCATAAAATCATAGCGATCTTTCAGCGCCTCGCTGGACCCCATC‑aaaaaaag                                     <  1:2579052/62‑3 (MQ=255) aCCTGGAATTTCATAAAATCATAGCGATCTTTCAGCGCCTCGCTGGACCCCATC‑aaaaaaag                                     <  1:2354063/62‑3 (MQ=255) aCCTGGAATTTCATAAAATCATAGCGATCTTTCAGCGCCTCGCTGGACCCCATC‑aaaaaaag                                     <  1:1646699/62‑3 (MQ=255) aCCTGGAATTTCATAAAATCATAGCGATCTTTCAGCGCCTCGCTGGACCCCATC‑aaaaaaag                                     <  1:1191147/62‑3 (MQ=255)                                      ccTCGCTGGACCCCATCAAAAAAAGCCATGATCcgc                           >  1:3027222/1‑36 (MQ=37)                                      ccTCGCTGGACCCCATCAAAAAAAGCCATGATCCGCGCGATGCCTGGGCCTCCATCTGAtt  >  1:1915515/1‑61 (MQ=255)                                      ccTCGCTGGACCCCATCAAAAAAAGCCATGATCCGCGCGATGCCTGGGCCTCCATCTGAtt  >  1:2036254/1‑61 (MQ=255)                                      ccTCGCTGGACCCCATCAAAAAAAGCCATGATCCGCGCGATGCCTGGGCCTCCATCTGAtt  >  1:2720666/1‑61 (MQ=255)                                      ccTCGCTGGACCCCATCAAAAAAAGCCATGATCCGCGCGATGCCTGGGCCTCCATCTGAtt  >  1:3050709/1‑61 (MQ=255)                                      ccTCGCTGGACCCCATCAAAAAAAGCCATGATCCGCGCGATGCCTGGGCCTCCATCTGAtt  >  1:309836/1‑61 (MQ=255)                                      ccTCGCTGGACCCCATCAAAAAAAGCCATGATCCGCGCGATGCCTGGGCCTCCATCTGAtt  >  1:40876/1‑61 (MQ=255)                                      ccTCGCTGGACCCCATCAAAAAAAGCCATGATCCGCGCGATGCCTGGGCCTCCATCTGAtt  >  1:1029260/1‑61 (MQ=255)                                      ccTCGCTGGACCCCATCAAAAAAAGCCATGATCCGCGCGATGCCTGGGCCTCCATCTGAtt  >  1:850562/1‑61 (MQ=255)                                      ccTCGCTGGACCCCATCAAAAAAAGCCATGATCCGCGCGATGCCTGGGCCTCCATCTGAt   >  1:1216829/1‑60 (MQ=255)                                                       |                                            ACCTGGAATTTCATAAAATCATAGCGATCTTTCAGCGCCTCGCTGGACCCCATC‑AAAAAAGCCATGATCCGCGCGATGCCTGGGCCTCCATCTGATT  >  W3110S.gb/1592992‑1593088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑