| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,976,768 | (A)5→6 | coding (271/1965 nt) | cheA ← | fused chemotactic sensory histidine kinase (soluble) in two‑component regulatory system with CheB and CheY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,976,763 | 1 | . | A | 83.3% | 10.4 / ‑2.0 | 6 | coding (276/1965 nt) | cheA | fused chemotactic sensory histidine kinase (soluble) in two‑component regulatory system with CheB and CheY |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (0/5); total (0/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGC > W3110S.gb/1976711‑1976823 | ctTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑AAAAACAGa < 1:2224326/62‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:325808/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:2623746/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:2646669/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:2647344/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:2687969/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:2715884/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:2780643/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:2928005/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:2964694/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:308896/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:1406555/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:332089/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:42273/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:4308/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:568377/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:736861/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:761919/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:943599/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:972242/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:979528/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:2371603/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:1495211/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:223573/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:222053/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:1930893/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:1904842/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:1684818/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:1625990/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:1797627/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:1771279/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:1730987/39‑1 (MQ=255) gTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:2605495/39‑1 (MQ=255) tCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑aaaaa < 1:771138/38‑1 (MQ=255) tGTTCTTGCATGATGTCCTTCGTTTCCAAAAAACAGATTGATAATGTc < 1:944736/48‑1 (MQ=255) tGTTCTTGCATGATGTCCTTCGTTTCCAAAAAACAGATTGATAATGTc < 1:869141/48‑1 (MQ=255) tGTTCTTGCATGATGTCCTTCGTTTCCAAAAAACAGATTGATAATGTc < 1:2066161/48‑1 (MQ=255) tGTTCTTGCATGATGTCCTTCGTTTCCAAAAAACAGATTGATAATGTc < 1:1167109/48‑1 (MQ=255) gTTCTTGCATGATGTCCTTCGTTTCCAAAAAACAGATTGATAATGTc < 1:164966/47‑1 (MQ=255) cc‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGc > 1:1726072/1‑62 (MQ=255) cc‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGc > 1:900680/1‑62 (MQ=255) cc‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGc > 1:958400/1‑62 (MQ=255) cc‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGc > 1:77016/1‑62 (MQ=255) cc‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGc > 1:623572/1‑62 (MQ=255) cc‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGc > 1:584981/1‑62 (MQ=255) cc‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGc > 1:1871351/1‑62 (MQ=255) cc‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGc > 1:189902/1‑62 (MQ=255) cc‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGc > 1:2657166/1‑62 (MQ=255) cc‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGc > 1:2495556/1‑62 (MQ=255) | CTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCC‑AAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGC > W3110S.gb/1976711‑1976823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |