Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	3454586	3454586	1	7 [0]	[0] 2	rpoB	RNA polymerase, beta subunit

GACTCATCAATATAGTCTTTAGCAACCACTTTACCTGCGATGTACTCAACCGGGACTTCGATC  >  W3110S.gb/3454523‑3454585                                                               | gACTCATCAATATAGTC‑‑TAGCAACCACTTTACCTGCGATGTACTCAACCGGGACTTCGATc  <  1:2563970/61‑1 (MQ=255)   cTCATCAATATAGTCTTTAGCAACCACTTTACCTGCGATGTACTCAACCGGGACTTCGATc  <  1:1039119/61‑1 (MQ=255)   cTCATCAATATAGTCTTTAGCAACCACTTTACCTGCGATGTACTCAACCGGGACTTCGATc  <  1:1047558/61‑1 (MQ=255)   cTCATCAATATAGTCTTTAGCAACCACTTTACCTGCGATGTACTCAACCGGGACTTCGATc  <  1:1148024/61‑1 (MQ=255)   cTCATCAATATAGTCTTTAGCAACCACTTTACCTGCGATGTACTCAACCGGGACTTCGATc  <  1:2291226/61‑1 (MQ=255)   cTCATCAATATAGTCTTTAGCAACCACTTTACCTGCGATGTACTCAACCGGGACTTCGATc  <  1:2373205/61‑1 (MQ=255)   cTCATCAATATAGTCTTTAGCAACCACTTTACCTGCGATGTACTCAACCGGGACTTCGATc  <  1:2528372/61‑1 (MQ=255)                                                               | GACTCATCAATATAGTCTTTAGCAACCACTTTACCTGCGATGTACTCAACCGGGACTTCGATC  >  W3110S.gb/3454523‑3454585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑