Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	133273	133273	1	4 [0]	[0] 4	acnB	bifunctional aconitate hydratase 2 and 2‑methylisocitrate dehydratase

CCGCTGTATGCGATCAAACAAGGTCTGCTGACCGTT  >  W3110S.gb/133274‑133309 |                                    ccGCTGTATGCGATCAAACAAGGTCTGCTGACCGtt  <  1:1851208/36‑1 (MQ=255) ccGCTGTATGCGATCAAACAAGGTCTGCTGACCGtt  <  1:2734267/36‑1 (MQ=255) ccGCTGTATGCGATCAAACAAGGTCTGCTGACCGtt  <  1:2944163/36‑1 (MQ=255) ccGCTGTATGCGATCAAACAAGGTCTGCTGACCGtt  <  1:356373/36‑1 (MQ=255) |                                    CCGCTGTATGCGATCAAACAAGGTCTGCTGACCGTT  >  W3110S.gb/133274‑133309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑