Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	3685461	3685478	18	4 [0]	[0] 12	ilvG	Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

AGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACG  >  W3110S.gb/3685479‑3685518 |                                        aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:1761057/1‑40 (MQ=255) aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:1863520/1‑40 (MQ=255) aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:234997/1‑40 (MQ=255) aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:2795936/1‑40 (MQ=255) aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:504820/1‑40 (MQ=255) aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:552957/1‑40 (MQ=255) aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:60812/1‑40 (MQ=255) aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:642808/1‑40 (MQ=255) aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:642897/1‑40 (MQ=255) aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:70155/1‑40 (MQ=255) aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:909489/1‑40 (MQ=255) aGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACg  >  1:914791/1‑40 (MQ=255) |                                        AGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACG  >  W3110S.gb/3685479‑3685518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑