Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	3685602	3685665	64	4 [0]	[1] 2	ilvG	Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

ATCGACCAGAACCGGACCAGGACGACCTGAGCAGGCAACGTCGAATGCTTCAGCCATGATGCGCGGCAACTC  >  W3110S.gb/3685656‑3685727           |                                                              atCGACCAGAACCGGACCAGGACGACCTGAGCAGGCAACGTCGAATGCTTCAGCCATGATgc            <  1:1151752/62‑1 (MQ=255)           aCCGGACCAGGACGACCTGAGCAGGCAACGTCGAATGCTTCAGCCATGATGCGCGGCAActc  <  1:2084419/62‑1 (MQ=255)           |                                                              ATCGACCAGAACCGGACCAGGACGACCTGAGCAGGCAACGTCGAATGCTTCAGCCATGATGCGCGGCAACTC  >  W3110S.gb/3685656‑3685727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑