| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,103,370 | 0 | A | C | 72.4% | 58.3 / 14.2 | 29 | P79P (CCT→CCG) | greB | transcription elongation factor |
| Reads supporting (aligned to +/- strand): ref base A (8/0); new base C (0/21); total (8/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGAGGGGAGTAATCGACGATTTTGAGATTTTCCAGGCATTTAGTGAGATA > W3110S.gb/4103321‑4103417 | cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:2559008/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:975657/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:886078/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:814968/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:773396/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:548415/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:531333/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:378100/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:2848304/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:2806719/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:2593232/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:1232065/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:2509595/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:2437661/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:2378287/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:2125094/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:1639701/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:1576299/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:1304471/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGCCGGGAGTAATc < 1:832261/61‑1 (MQ=255)cgtTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGAGGGGAGTAATcg > 1:1265269/1‑62 (MQ=255) tcCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGAGGGGAGTAATCGACGATTTCgag > 1:159209/1‑62 (MQ=255) cAAAAAAGACTTTGCCTTCCTGCTGCGGGGAGTAATc < 1:347495/37‑1 (MQ=255) ttGCCTTCCTGCTTAGGGGAGTAATCGACGATTTTGAGATTTTCCAGGCATTTAGTGAGATa > 1:956841/1‑62 (MQ=255) ttGCCTTCCTGCTGAGGGGAGTAATCGACGATTTTGAGATTTTCCAGGCAttt > 1:429196/1‑53 (MQ=255) ttGCCTTCCTGCTGAGGGGAGTAATCGACGATTTTGAGATTTTCCAGGCATTTAGTGAGATa > 1:1994677/1‑62 (MQ=255) ttGCCTTCCTGCTGAGGGGAGTAATCGACGATTTTGAGATTTTCCAGGCATTTAGTGAGATa > 1:1773732/1‑62 (MQ=255) ttGCCTTCCTGCTGAGGGGAGTAATCGACGATTTTGAGATTTTCCAGGCATTTAGTGAGATa > 1:1361885/1‑62 (MQ=255) ttGCCTTCCTGCTGAGGGGAGTAATCGACGATTTTGAGATTTTCCAGGCATTTAGTGAGATa > 1:1288720/1‑62 (MQ=255) | CGTTTTCAATCTCCACCCACGCGCCAAAAAAGACTTTGCCTTCCTGCTGAGGGGAGTAATCGACGATTTTGAGATTTTCCAGGCATTTAGTGAGATA > W3110S.gb/4103321‑4103417 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |