| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,457,719 | 0 | C | A | 72.2% | 18.1 / 10.5 | 18 | A155D (GCC→GAC) | ytfT | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/5); new base A (0/13); total (0/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATG > W3110S.gb/4457661‑4457758 | tttGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCgg < 1:3068800/62‑1 (MQ=255)tttGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCgg < 1:1844303/62‑1 (MQ=255)tttGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCgg < 1:217101/62‑1 (MQ=255)tttGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGAACACCGCCgg < 1:772640/62‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:1365100/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:839165/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:2895941/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:2708737/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:2708432/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:2655365/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:2143036/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:2129718/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:20525/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:1718100/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:1587618/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:1199813/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCGGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc < 1:772625/51‑1 (MQ=255) gcgTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATg < 1:2390815/61‑1 (MQ=255) | TTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATG > W3110S.gb/4457661‑4457758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |