| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 23,325 | 0 | G | A | 73.1% | 36.4 / 14.1 | 26 | G312D (GGC→GAC) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (4/3); new base A (0/19); total (4/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.34e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTAC > W3110S.gb/23264‑23376 | aTTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATggg < 1:746132/62‑1 (MQ=255)aTTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATggg < 1:696943/62‑1 (MQ=255)aTTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATggg < 1:165337/62‑1 (MQ=255)aTTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATggg < 1:1516410/62‑1 (MQ=255)aTTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATggg < 1:2302152/62‑1 (MQ=255)aTTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATggg < 1:1446730/62‑1 (MQ=255)aTTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATggg < 1:366856/62‑1 (MQ=255)aTTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATggg < 1:3110184/62‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:382588/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:426343/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:3082222/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:3099611/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:3099877/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:434232/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:2220141/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:510366/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:54066/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:3117331/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:563857/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:1997163/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:717331/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:1259069/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:1195182/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:1408403/61‑1 (MQ=255) aGGTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:168927/61‑1 (MQ=255) ggTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:1981634/60‑1 (MQ=255) ggTGCCGAGCTGGAACTGTTGCGCTTTACCCATCCGTTTATGGACTTCGATGTTCCGGCa < 1:718781/60‑1 (MQ=255) gCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATggg < 1:2645405/41‑1 (MQ=255) gAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCgg < 1:1830028/62‑1 (MQ=255) gAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCgg < 1:1680117/62‑1 (MQ=255) aCCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCgg < 1:534118/41‑1 (MQ=255) ccGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTAc > 1:487275/1‑62 (MQ=255) ccGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTAc > 1:149442/1‑62 (MQ=255) ccGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTAc > 1:2103074/1‑62 (MQ=255) ccGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTAc > 1:1299252/1‑62 (MQ=255) tggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:1290096/44‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:2240010/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:668561/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:734683/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:659876/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:2139900/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:1092510/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:1301535/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:1343902/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:1611305/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:1996388/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:2031833/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:2124809/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:331868/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:218693/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:2208640/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:1038150/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:224583/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:2755668/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:2826843/45‑1 (MQ=255) gggCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:2899391/45‑1 (MQ=255) gCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGa < 1:1857899/43‑1 (MQ=255) | ATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTAC > W3110S.gb/23264‑23376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |