| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 63,948 | 0 | G | A | 73.9% | 32.8 / 10.7 | 23 | T611T (ACC→ACT) | polB | DNA polymerase II |
| Reads supporting (aligned to +/- strand): ref base G (0/6); new base A (17/0); total (17/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.91e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACACCATCCGCTGCTTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATGGGTTTCATA > W3110S.gb/63889‑64001 | aaCACCATCCGCTGCTTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCgg < 1:672084/61‑1 (MQ=255)aaCACCATCCGCTGCTTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCgg < 1:190691/61‑1 (MQ=255)aaCACCATCCGCTGCTTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCgg < 1:2605965/61‑1 (MQ=255) aGTGCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:363943/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGGCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:1346438/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGTCATCAGAAAAc > 1:1160028/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:891212/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:1069032/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:784398/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:722324/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:374776/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:368154/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:2933827/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:2678174/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:2625848/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:2159622/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:1655174/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:1448618/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:1440962/1‑61 (MQ=255) aGTCCGGCATAACGCTTTTTACTGCCAGTATCGGCTCCGCGAATGGTTGGCATCAGAAAAc > 1:1180095/1‑61 (MQ=255) tACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATGGGTTTCATa < 1:2884113/61‑1 (MQ=255) tACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATGGGTTTCATa < 1:1505298/61‑1 (MQ=255) tACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATGGGTTTCATa < 1:428311/61‑1 (MQ=255) tACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATGGGTTTCATa < 1:660103/61‑1 (MQ=255) tACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATGGGTTTCATa < 1:1321207/61‑1 (MQ=255) tACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATGGGTTTCATa < 1:840314/61‑1 (MQ=255) | AACACCATCCGCTGCTTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATGGGTTTCATA > W3110S.gb/63889‑64001 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |