| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 316,457 | 0 | G | A | 71.9% | 44.4 / 19.4 | 32 | intergenic (‑97/+493) | ykgA/ykgB | predicted DNA‑binding transcriptional regulator/conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (2/7); new base A (23/0); total (25/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCCCTAAGTGTATCAAAAATAGCAAGGGCGAAGGCAAAAGCACTGGTAGCGTCTGCTTCGTTGTCCCCGAAAATAGAGAGGTTTCAGTCC > W3110S.gb/316410‑316501 | gggCCCTGAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:2254090/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTg > 1:336758/1‑57 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTg > 1:3085904/1‑57 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTg > 1:188230/1‑57 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTg > 1:272920/1‑57 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:107117/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:860088/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:65976/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:645247/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:3086497/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:3031352/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:2960367/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:2708215/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:2678977/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:2437684/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:2313092/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:1040124/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:2265769/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:2074546/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:1993373/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:117757/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:1392269/1‑58 (MQ=255)gggCCCTAAGTGTATCAAAAACAGCAAGTCCCAAGGCAAAAGCACTGATAGCGTCTGc > 1:1452216/1‑58 (MQ=255) aaGGGCGAAGGCAAAAGCACTGGTAGCGTCTGCTTCGTTGTCCCCGAAAATAGAGAGGTTTc < 1:2559243/62‑1 (MQ=255) aaGGGCGAAGGCAAAAGCACTGGTAGCGTCTGCTTCGTTGTCCCCGAAAATAGAGAGGTTTc < 1:807300/62‑1 (MQ=255) aaGGGCGAAGGCAAAAGCACTGGTAGCGTCTGCTTCGTTGTCCCCGAAAATAGAGAGGTTTc < 1:456579/62‑1 (MQ=255) aaGGGCGAAGGCAAAAGCACTGGTAGCGTCTGCTTCGTTGTCCCCGAAAATAGAGAGGTTTc < 1:1714727/62‑1 (MQ=255) aaGGGCGAAGGCAAAAGCACTGGTAGCGTCTGCTTCGTTGTCCCCGAAAATAGAGAGGTTTc < 1:2564317/62‑1 (MQ=255) aGGGCGAAGGCAAAAGCACTGGTAGCGTCTGCTTCGTTGTCCCCGAAAATAGAGAGGTTTc < 1:1262691/61‑1 (MQ=255) cGAAGGCAAAAGCACTGGTAGCGTCTGCTTCGTTGTCCCCGAAAATAGAGAGGTTTCAGTcc > 1:177035/1‑62 (MQ=255) cGAAGGCAAAAGCACTGGTAGCGTCTGCTTCGTTGTCCCCGAAAATAGAGAGGTTTCAGTc > 1:1876664/1‑61 (MQ=255) ggCAAAAGCACTGGTAGCGTCTGCTTCGTTGTCCCCGAAAATAGAGAGGTTTc < 1:2698279/53‑1 (MQ=255) | GGGCCCTAAGTGTATCAAAAATAGCAAGGGCGAAGGCAAAAGCACTGGTAGCGTCTGCTTCGTTGTCCCCGAAAATAGAGAGGTTTCAGTCC > W3110S.gb/316410‑316501 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |