| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 429,106 | 0 | A | G | 73.6% | 87.3 / 31.9 | 53 | A126A (GCA→GCG) | secF | SecYEG protein translocase auxillary subunit |
| Reads supporting (aligned to +/- strand): ref base A (14/0); new base G (39/0); total (53/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGT > W3110S.gb/429049‑429166 | tCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGcagcag < 1:2209018/62‑1 (MQ=255) cAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGcagcag < 1:929629/61‑1 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtcg > 1:273900/1‑62 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtcg > 1:2092024/1‑62 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtcg > 1:1930978/1‑62 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtcg > 1:1518110/1‑62 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtcg > 1:496797/1‑62 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtcg > 1:2731468/1‑62 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtcg > 1:1244963/1‑62 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtcg > 1:2388140/1‑62 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtcg > 1:121254/1‑62 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtcg > 1:2673268/1‑62 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtcg > 1:1182456/1‑62 (MQ=255) tCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgtc > 1:527560/1‑61 (MQ=255) gATTAACGAATCCACCAATTAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:1637938/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTc > 1:2644915/1‑56 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGc > 1:958702/1‑61 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:356846/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:2855985/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:2915635/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:3087213/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:3087715/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:3106852/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:3131539/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:323943/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:976546/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:39201/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:614801/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:707616/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:821480/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:895616/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:1054094/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:1244709/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:1381750/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:2466905/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:1643137/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:1809601/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:1088548/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:1963451/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:2132718/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:1107780/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:1326016/1‑62 (MQ=255) gATTAACGAATCCACCAATCAGAATGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg > 1:2253219/1‑62 (MQ=255) aTTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGc > 1:2703411/1‑60 (MQ=255) aTTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGt > 1:1887375/1‑62 (MQ=255) aTGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCtt > 1:14532/1‑53 (MQ=255) aTGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAAcc > 1:1376904/1‑62 (MQ=255) aTGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAAcc > 1:560840/1‑62 (MQ=255) aTGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAAcc > 1:926469/1‑62 (MQ=255) aTGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAAcc > 1:1200599/1‑62 (MQ=255) aTGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAAcc > 1:2038746/1‑62 (MQ=255) aTGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAAcc > 1:2109575/1‑62 (MQ=255) aTGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAAcc > 1:2202904/1‑62 (MQ=255) aTGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAAcc > 1:2759554/1‑62 (MQ=255) aTGCGGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAAc > 1:2380223/1‑61 (MQ=255) agcagTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTg > 1:60623/1‑50 (MQ=255) agcagTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGt > 1:1064080/1‑61 (MQ=255) agcagTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGt > 1:477214/1‑61 (MQ=255) agcagTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGt > 1:720052/1‑61 (MQ=255) agcagTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGt > 1:62752/1‑61 (MQ=255) agcagTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGt > 1:1905959/1‑61 (MQ=255) agcagTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGt > 1:2682561/1‑61 (MQ=255) | TCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGT > W3110S.gb/429049‑429166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |