Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	705,013	0	T	C	72.7%	30.2 / 10.9	22	I216I (ATT→ATC)	nagE	fused N‑acetyl glucosamine specific PTS enzyme IICBA components
Reads supporting (aligned to +/- strand):  ref base T (6/0);  new base C (16/0);  total (22/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGG  >  W3110S.gb/704961‑705045                                                     |                                 tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:1251297/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:2695600/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:2529427/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:2456604/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:2236472/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:379022/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:2117937/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:2097737/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:2088326/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:203445/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:1553610/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:1508219/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:1491700/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:1470868/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:1238168/1‑62 (MQ=37) tCCAGATTGGTGAATTCACCAACGCGCCGGGAACGGTTTTCCACGGTGATATCAACCGCTTc                         >  1:329197/1‑62 (MQ=37)                        gcggcgGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCggg  >  1:763812/1‑62 (MQ=255)                        gcggcgGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCggg  >  1:3080923/1‑62 (MQ=255)                        gcggcgGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCggg  >  1:3003128/1‑62 (MQ=255)                        gcggcgGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCggg  >  1:2509950/1‑62 (MQ=255)                        gcggcgGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCggg  >  1:2199046/1‑62 (MQ=255)                        gcggcgGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCggg  >  1:1299204/1‑62 (MQ=255)                                                     |                                 TCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGG  >  W3110S.gb/704961‑705045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑