| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 958,895 | 0 | A | T | 71.9% | 48.2 / 19.9 | 32 | E274V (GAA→GTA) | serC | 3‑phosphoserine/phosphohydroxythreonine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (9/0); new base T (0/23); total (9/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGATTTCTA > W3110S.gb/958835‑958931 | aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGa < 1:1176150/61‑1 (MQ=255)aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGa < 1:1256695/61‑1 (MQ=255)aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGa < 1:674886/61‑1 (MQ=255)aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGa < 1:2399704/61‑1 (MQ=255)aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGa < 1:606629/61‑1 (MQ=255)aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGa < 1:335550/61‑1 (MQ=255)aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGAAAAAATCAATCAGCAAAAAGCAGa < 1:215668/61‑1 (MQ=255) cTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGc > 1:1065928/1‑61 (MQ=255) cTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGc > 1:2850001/1‑61 (MQ=255) cTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGc > 1:1657150/1‑61 (MQ=255) cTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGc > 1:583944/1‑61 (MQ=255) cTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGc > 1:2218944/1‑61 (MQ=255) gCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGa < 1:102978/50‑1 (MQ=255) tGAAATGGATAAAATCAATCAGCAATAAGCAGAACTGCTATATGGGGtg > 1:452884/1‑49 (MQ=255) tGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCg > 1:2768449/1‑62 (MQ=255) tGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCg > 1:2768450/1‑62 (MQ=255) aaaTGGATAAAATCAATCAGCAAAAAGCAGAACTGc > 1:2209016/1‑36 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:392452/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:395712/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:541837/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:32093/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:672643/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:673882/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:1007442/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:778471/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:2497337/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:243048/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:238686/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:2352241/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:2327580/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:2196517/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:2188513/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:2070970/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:184834/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:180344/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:1735905/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:1551860/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:1436932/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:1433477/42‑1 (MQ=255) taGCAGTTCTGCTATATGGGGTGATTGATAACAGCGATTTCTa < 1:1092250/42‑1 (MQ=255) | AATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGATTTCTA > W3110S.gb/958835‑958931 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |