| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,099,635 | 0 | C | T | 73.2% | 87.6 / 36.0 | 56 | N172N (AAC→AAT) | ycdX | predicted zinc‑binding hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (6/9); new base T (41/0); total (47/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGAT > W3110S.gb/1099594‑1099695 | aatTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGc < 1:2835605/62‑1 (MQ=255) tCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGt > 1:2395030/1‑62 (MQ=255) tCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGt > 1:2627462/1‑62 (MQ=255) tCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGt > 1:667049/1‑62 (MQ=255) tCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGt > 1:1861712/1‑62 (MQ=255) tCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGt > 1:2924853/1‑62 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCTCAGCGGTAc > 1:140272/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTa > 1:2297835/4‑60 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTa > 1:2255449/4‑60 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2949410/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2624875/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2638749/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2806541/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2897206/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2901583/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2930692/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1198810/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:3061170/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:332193/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:338335/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:352397/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:596086/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:620398/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:705636/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:745218/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:774053/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:800145/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:87954/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2330521/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1139724/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1334464/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1340573/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1402019/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1472784/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1504986/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1670399/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1757122/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1788344/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1799087/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:1945063/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:187971/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2098987/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2171843/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2375591/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:2272482/4‑61 (MQ=255) tcttttttACACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGAAGCGGTAc > 1:885845/4‑61 (MQ=255) tcttttttAAACTCACGTAAGGGAAGTGAAGACAATTGTCGTGCGGTAGCCGCAGCGGTAc > 1:133742/4‑61 (MQ=255) gACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTc < 1:532374/62‑1 (MQ=255) gACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTc < 1:1563390/62‑1 (MQ=255) gACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTc < 1:2782611/62‑1 (MQ=255) gACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTc < 1:2488046/62‑1 (MQ=255) gACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTc < 1:16522/62‑1 (MQ=255) gACAACTGTCGTGAGGTAGACGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTc < 1:219574/62‑1 (MQ=255) aCAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTc < 1:1776307/61‑1 (MQ=255) aCAACTGGCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTc < 1:2186452/61‑1 (MQ=255) aCTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGAt > 1:1632930/1‑62 (MQ=255) | AATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGAT > W3110S.gb/1099594‑1099695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |