| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,247,586 | 0 | T | C | 71.9% | 86.9 / 38.6 | 57 | L456L (TTA→TTG) | treA | periplasmic trehalase |
| Reads supporting (aligned to +/- strand): ref base T (7/9); new base C (0/41); total (7/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCG > W3110S.gb/1247529‑1247635 | gTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAAc < 1:3058144/61‑1 (MQ=255)gTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAAc < 1:2364312/61‑1 (MQ=255)gTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAAc < 1:1515807/61‑1 (MQ=255)gTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAAc < 1:2481398/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcg < 1:389370/61‑2 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2644477/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2162822/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:388472/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2298620/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2379346/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2427367/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:3079398/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2073319/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2649072/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2737893/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2744517/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2761196/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2791008/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2976053/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2914902/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1622642/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1089550/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:942446/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:884333/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:739635/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1388881/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1403726/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1519034/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:723146/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:602806/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2083266/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:575701/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1704512/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:177353/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:465131/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1821548/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1888062/61‑1 (MQ=255) cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:190962/61‑1 (MQ=255) gCCACCTCTTTTTGCCCGTAGTTTTTTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1183846/60‑1 (MQ=255) gCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1196980/60‑1 (MQ=255) ttttGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:3068060/51‑1 (MQ=255) tttGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2267652/50‑1 (MQ=255) gCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1580790/47‑1 (MQ=255) gTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATccc > 1:2793143/1‑62 (MQ=255) gTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATccc > 1:132532/1‑62 (MQ=255) gTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATccc > 1:2451798/1‑62 (MQ=255) gTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATccc > 1:180221/1‑62 (MQ=255) gTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATccc > 1:1651875/1‑62 (MQ=255) gTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATccc > 1:153609/1‑62 (MQ=255) tAGTTTTGTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:1270252/42‑1 (MQ=255) gTAATCCTTCTGTCGCGACCCACTGCAACGGTGcc < 1:2964326/35‑1 (MQ=255) cgcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCg > 1:369710/1‑62 (MQ=255) gcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCAc < 1:2135644/59‑1 (MQ=255) gcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCAc < 1:635576/59‑1 (MQ=255) gcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCAc < 1:3063395/59‑1 (MQ=255) gcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCAc < 1:3018473/59‑1 (MQ=255) gcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCAc < 1:2830888/59‑1 (MQ=255) | GTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCG > W3110S.gb/1247529‑1247635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |