Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,902,991	0	G	A	72.7%	29.4 / 11.6	22	T103T (ACC→ACT)	yoaE	fused predicted membrane proteins
Reads supporting (aligned to +/- strand):  ref base G (0/6);  new base A (16/0);  total (16/6)
Fisher's exact test for biased strand distribution p-value = 1.34e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATATCCCCCCGAACAACATAATCA  >  W3110S.gb/1902944‑1903037                                                |                                               ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGcagttcagtt                                             >  1:122564/1‑51 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:67559/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:551260/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:852430/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:3028943/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:2684822/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:2668327/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:2556250/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:2024817/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:2010745/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:189961/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:1835879/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:1809372/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:1774669/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:1612621/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:1537220/1‑60 (MQ=255) ccGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCAGTTGTTGCTTTg                                    >  1:1531309/1‑60 (MQ=255)  cGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGaa                                  <  1:2453634/61‑1 (MQ=255)  cGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGaa                                  <  1:930421/61‑1 (MQ=255)                     gcgGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATATCCCCCCGa              <  1:1286687/62‑1 (MQ=255)                     gcgGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATATCCCCCCGa              <  1:3127606/62‑1 (MQ=255)                                 ccGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATATCCCCCCGAACAACATAATCa  <  1:2920756/62‑1 (MQ=255)                                 ccGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATATCCCCCCGAACAACATAATCa  <  1:996394/62‑1 (MQ=255)                                                |                                               CCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATATCCCCCCGAACAACATAATCA  >  W3110S.gb/1902944‑1903037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑