Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,690,9180GA73.0% 54.9 / 22.4 37F1094F (TTC→TTT) purLphosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (2/8);  new base A (27/0);  total (29/8)
Fisher's exact test for biased strand distribution p-value = 1.17e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGGATTGACTTCGCCCAACCTTCACCGGCACCCAGCACATCACCGTAGGAGAAACCACCGCACGCGACCAGGGCGTGGAAATCTTCCAGGCCCGTGCGTCCGGT  >  W3110S.gb/2690867‑2690971
                                                   |                                                     
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCg                                              >  1:1032445/1‑61 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCg                                              >  1:1847000/1‑61 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCg                                              >  1:2749035/1‑61 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCg                                              >  1:1709249/1‑61 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCg                                              >  1:2952443/1‑61 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCg                                              >  1:1373197/1‑61 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCg                                              >  1:605252/1‑61 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCg                                              >  1:1126663/1‑61 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:423052/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:361604/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:315072/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:676693/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:2937666/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:289981/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:734223/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:2885683/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:1938792/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:1933282/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:103524/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:176332/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:1186330/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:1415709/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:1553275/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:1601102/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:1724060/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATCACCGTAGGAAAAACCACCGc                                             >  1:1726956/1‑62 (MQ=255)
cAGGATAGACTTCGCCCAACCTTCACCAGCGCCCAGCACATAACCGTAGGAAAAACCACCg                                              >  1:137687/1‑61 (MQ=255)
       gACTTCGCCCAACCTTCACCGGCACCCAGCACATCACCGTAGGAGAAACCACCGCAcgcg                                        >  1:1176536/1‑60 (MQ=255)
                cAACCTTCACCGGCACCCAGCACATCACCGTAGGAGAAACCACCGCACGCGACCAGGGCggg                             <  1:1934193/62‑3 (MQ=255)
                cAACCTTCACCGGCACCCAGCACATCACCGTAGGAGAAACCACCGCACGCGACCAGGGCGTg                             <  1:2313353/62‑1 (MQ=255)
                cAACCTTCACCGGCACCCAGCACATCACCGTAGGAGAAACCACCGCACGCGACCAGGGCGTg                             <  1:1371459/62‑1 (MQ=255)
                 aaCCTTCACCGGCACCCAGCACATCACCGTAGGAGAAACCACCGCACGCGACCAGGGCGTg                             <  1:2050186/61‑1 (MQ=255)
                 aaCCTTCACCGGCACCCAGCACATCACCGTAGGAGAAACCACCGCACGCGACCAGGGCGTg                             <  1:1838572/61‑1 (MQ=255)
                 aaCCTTCACCGGCACCCAGCACATCACCGTAGGAGAAACCACCGCACGCGACCAGGGCGTg                             <  1:709442/61‑1 (MQ=255)
                                            cGTAGGAGAAACCACCGCACGCGACCAGGGCGTGGAAATCTTCCAGGCCCGTGCGTCCGGt  >  1:1643913/1‑61 (MQ=255)
                                            cGTAGGAGAAACCACCGCACGCGACCAGGGCGTGGAAATCTTCCAGGCCCGTGCGTCCGGt  <  1:1757915/61‑1 (MQ=255)
                                            cGTAGGAGAAACCACCGCACGCGACCAGGGCGTGGAAATCTTCCAGGCCCGTGCGTCCGGt  <  1:1468826/61‑1 (MQ=255)
                                                   |                                                     
CAGGATTGACTTCGCCCAACCTTCACCGGCACCCAGCACATCACCGTAGGAGAAACCACCGCACGCGACCAGGGCGTGGAAATCTTCCAGGCCCGTGCGTCCGGT  >  W3110S.gb/2690867‑2690971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑