| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,680,100 | 0 | T | C | 73.7% | 58.6 / 21.4 | 38 | L417L (CTA→CTG) | ilvA | threonine deaminase |
| Reads supporting (aligned to +/- strand): ref base T (6/4); new base C (0/28); total (6/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.61e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGCGATGGACGTCCGCCGACCATATAGCGCACGTGTAGCTTCGCCATTTCGTCGTCGGAGAGATCAACCACGCTGTAGCCGCCGTCGTTGAGC > W3110S.gb/3680064‑3680157 | aTGCGATGGACGTCCGCCGACCATATAGCGCACGTGTAGCTTCGCCATTTCGTCGTCGgaga < 1:270738/62‑1 (MQ=255) gCGATGGACGTCCGCCGACCATATAGCGCACGTGTAGCTTCGCCATTTCGTCGTCGgagaga < 1:2564957/62‑1 (MQ=255) gCGATGGACGTCCGCCGACCATATAGCGCACGTGTAGCTTCGCCATTTCGTCGTCGgagaga < 1:2588050/62‑1 (MQ=255) gCGATGGACGTCCGCCGACCATATAGCGCACGTGTAGCTTCGCCATTTCGTCGTCGgagaga < 1:2234844/62‑1 (MQ=255) gccgACCATATAGCGCACGTGTAGCTTCGCCATTTCGTCGTCGGAGAGATCAACCACGCTGt > 1:1161914/1‑62 (MQ=255) gccgACCATATAGCGCACGTGTAGCTTCGCCATTTCGTCGTCGGAGAGATCAACCACGCTGt > 1:351397/1‑62 (MQ=255) gccgACCATATAGCGCACGTGTAGCTTCGCCATTTCGTCGTCGGAGAGATCAACCACGCTGt > 1:298267/1‑62 (MQ=255) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:252932/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:923263/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:2620943/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:2629773/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:2757118/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:2839618/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:2884248/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:331570/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:469961/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:713338/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:859617/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:1086693/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:251089/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:1258613/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:129774/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:1410935/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:1438553/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:1539144/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:1691464/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:1695017/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:1740407/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:17487/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:1759219/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:1964011/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:2217352/43‑1 (MQ=38) gACCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:2518783/43‑1 (MQ=38) aCCATATAGCGTACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:554074/42‑1 (MQ=37) aCCATATAGCGCACATGCAGCTTCGCCATTTCGTCGTCGgag < 1:696329/42‑1 (MQ=38) cGTGTAGCTTCGCCATTTCGTCGTCGGAGAGATCAACCACGCTGTAGCCGCCGTCGTTGAGc > 1:2663082/1‑62 (MQ=255) cGTGTAGCTTCGCCATTTCGTCGTCGGAGAGATCAACCACGCTGTAGCCGCCGTCGTTGAGc > 1:2453878/1‑62 (MQ=255) cGTGTAGCTTCGCCATTTCGTCGTCGGAGAGATCAACCACGATGTAGCCGCCGTCGTTGAGc > 1:1174695/1‑62 (MQ=255) | ATGCGATGGACGTCCGCCGACCATATAGCGCACGTGTAGCTTCGCCATTTCGTCGTCGGAGAGATCAACCACGCTGTAGCCGCCGTCGTTGAGC > W3110S.gb/3680064‑3680157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |