| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 556,858 | A→T | L36Q (CTG→CAG) | folD ← | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 556,858 | 0 | A | T | 100.0% | 95.0 / NA | 30 | L36Q (CTG→CAG) | folD | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (20/10); total (20/10) | |||||||||||
CTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTC > W3110S.gb/556807‑556913 | cttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACATCGGCCTGTCCTGGTGc > 1:1088186/1‑62 (MQ=255)cttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGc > 1:851891/1‑62 (MQ=255)cttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGc > 1:1451262/1‑62 (MQ=255)cttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCATGGTGc > 1:1149227/1‑62 (MQ=255) aCATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCa < 1:610143/61‑1 (MQ=255) aCATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCa < 1:627537/61‑1 (MQ=255) tGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt > 1:2449306/1‑37 (MQ=255) tGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAc > 1:1215213/1‑62 (MQ=255) tGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAc > 1:2653797/1‑62 (MQ=255) tGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAc > 1:2095420/1‑62 (MQ=255) cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc > 1:2903505/1‑62 (MQ=255) cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc > 1:761321/1‑62 (MQ=255) cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc > 1:786911/1‑62 (MQ=255) cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc > 1:1316689/1‑62 (MQ=255) cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcg > 1:1779221/1‑61 (MQ=255) ccaccaGCACAACGGCCTGTCCTGGTGCCCGCAGTcc > 1:665978/1‑37 (MQ=255) ccaccaGCACAACGGCCTGTCCTGGTGCCCGCAGTcc > 1:720877/1‑37 (MQ=255) ccaccaGCACAACGGCCTGTCCTGGTGCCCGCAGTcc > 1:393145/1‑37 (MQ=255) ccaccaGCACAACGGCCTGTCCTGGTGCCCGCAGTcc > 1:2486835/1‑37 (MQ=255) ccaccaGCACAACGGCCTGTCCTGGTGCCCGCAGTcc > 1:2668887/1‑37 (MQ=255) ccaccaGCACAACGGCCTGTCCTGGTGCCCGCAGTcc > 1:2048453/1‑37 (MQ=255) ccaccaGCACAACGGCCTGTCCTGGTGCCCGCAGTcc > 1:1861525/1‑37 (MQ=255) aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTc < 1:37747/62‑1 (MQ=255) aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTc < 1:2426553/62‑1 (MQ=255) aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTc < 1:2158686/62‑1 (MQ=255) aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTc < 1:726702/62‑1 (MQ=255) aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTc < 1:1712817/62‑1 (MQ=255) aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTc < 1:1346371/62‑1 (MQ=255) aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTc < 1:1204286/62‑1 (MQ=255) aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTc < 1:962633/62‑1 (MQ=255) | CTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTC > W3110S.gb/556807‑556913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |