Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,089,510	G→A	G170S (GGT→AGT)	endA →	DNA‑specific endonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,089,510	0	G	A	100.0%	90.2 / NA	28	G170S (GGT→AGT)	endA	DNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/18);  total (10/18)

TGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTC  >  W3110S.gb/3089453‑3089567                                                          |                                                          tGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTg                                                        >  1:1530710/1‑61 (MQ=255) tGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGc                                                       >  1:2340306/1‑62 (MQ=255)     ccATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    <  1:838800/61‑1 (MQ=255)     ccATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    <  1:2848686/61‑1 (MQ=255)     ccATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    <  1:2716130/61‑1 (MQ=255)     ccATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    <  1:1819355/61‑1 (MQ=255)     ccATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    <  1:1922522/61‑1 (MQ=255)     ccATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    <  1:2132106/61‑1 (MQ=255)     ccATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    <  1:2230245/61‑1 (MQ=255)             gTCGATTTCAAAGAAAAAGCTGCGGAACCACCAGCGCGTGCACGCAGTGCCATTGCGCGCAc                                           <  1:2655613/62‑1 (MQ=255)             gTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCATTGCGCGCAc                                           <  1:1235044/62‑1 (MQ=255)             gTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCATTGCGCGCAc                                           <  1:762663/62‑1 (MQ=255)             gTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCATTGCGCGCAc                                           <  1:612507/62‑1 (MQ=255)             gTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCATTGCGCGCAc                                           <  1:336058/62‑1 (MQ=255)             gTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCATTGCGCGCAc                                           <  1:254968/62‑1 (MQ=255)             gTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCATTGCGCGCAc                                           <  1:1285014/62‑1 (MQ=255)             gTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCATTGCGCGCAc                                           <  1:1064279/62‑1 (MQ=255)                     cAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    >  1:2231698/1‑45 (MQ=255)                     cAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    >  1:1987550/1‑45 (MQ=255)                     cAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    >  1:2637579/1‑45 (MQ=255)                     cAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    >  1:1714479/1‑45 (MQ=255)                     cAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    >  1:2686209/1‑45 (MQ=255)                     cAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    >  1:1533447/1‑45 (MQ=255)                     cAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    >  1:1435378/1‑45 (MQ=255)                     cAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCAt                                                    >  1:945302/1‑45 (MQ=255)                          aaaaaGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCATTGCGCGCACCTACTTCTATATg                              <  1:2110361/62‑1 (MQ=255)                          aaaaaGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCATTGCGCGCACCTACTTCTATATg                              <  1:1440138/62‑1 (MQ=255)                          aaaaaGCTGCCGAACCACCAGCGCGTGCACGCAGTGCCATTGCGCGCACCTACTTCTATATg                              <  1:724786/62‑1 (MQ=255)                                                       cgcaGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACtctctc  <  1:1596709/61‑1 (MQ=255)                                                          |                                                          TGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTC  >  W3110S.gb/3089453‑3089567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑