Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,381,305	C→T	G236G (GGC→GGT)	degQ →	serine endoprotease, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,381,305	0	C	T	100.0%	70.7 / NA	25	G236G (GGC→GGT)	degQ	serine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/23);  total (2/23)

GGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGC  >  W3110S.gb/3381276‑3381361                              |                                                         ggCATCAACACTGCAATCCTTGCGCCTGGTGGCGGGa                                                   <  1:751132/37‑1 (MQ=255) ggCATCAACACTGCAATCCTTGCGCCTGGTGGCGGGa                                                   <  1:1536645/37‑1 (MQ=255)       aaCACTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCAt                             <  1:1141428/53‑1 (MQ=255)       aaCACTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCAt                             <  1:377796/53‑1 (MQ=255)       aaCACTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCAt                             <  1:315670/53‑1 (MQ=255)       aaCACTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCAt                             <  1:310366/53‑1 (MQ=255)       aaCACTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCAt                             <  1:2712822/53‑1 (MQ=255)       aaCACTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCAt                             <  1:2625333/53‑1 (MQ=255)       aaCACTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCAt                             <  1:2613019/53‑1 (MQ=255)       aaCACTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCAt                             <  1:2167024/53‑1 (MQ=255)       aaCACTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCAt                             <  1:2163234/53‑1 (MQ=255)       aaCACTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCAt                             <  1:1232064/53‑1 (MQ=255)       aaCACTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCAt                             <  1:1023894/53‑1 (MQ=255)        acacTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAat                   >  1:2090145/1‑62 (MQ=255)           cTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATg                <  1:2219947/62‑1 (MQ=255)           cTGCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATg                <  1:449166/62‑1 (MQ=255)             gCAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATcc                           <  1:2636346/49‑1 (MQ=255)              cAATCCTTGCGCCTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGt                      >  1:497013/1‑53 (MQ=255)                         ccTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGgc  <  1:1867360/62‑1 (MQ=255)                         ccTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGgc  <  1:2875866/62‑1 (MQ=255)                         ccTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGgc  <  1:1744228/62‑1 (MQ=255)                         ccTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGgc  <  1:1176889/62‑1 (MQ=255)                         ccTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGgc  <  1:519364/62‑1 (MQ=255)                         ccTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGgc  <  1:76652/62‑1 (MQ=255)                         ccTGGTGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGgc  <  1:91582/62‑1 (MQ=255)                              |                                                         GGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGC  >  W3110S.gb/3381276‑3381361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑