| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,492,265 | T→C | N141S (AAC→AGC) | pflD ← | predicted formate acetyltransferase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,492,265 | 0 | T | C | 79.0% | 34.8 / 4.5 | 19 | N141S (AAC→AGC) | pflD | predicted formate acetyltransferase 2 |
| Reads supporting (aligned to +/- strand): ref base T (4/0); new base C (15/0); total (19/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
CCTTTATCCGTCTGGTTGATGCTGAAAATCTGCGTGTTGGTCGCGGCTTTTACTTCATCTGTCATCTGCCCGTTGATGAAATCTTTCATCGAACGTTT > W3110S.gb/3492229‑3492326 | ccTTTATCCGTCTGGTTGATGCTGAAAATCTGCGTGTTGGTCGCGGCTTTTACTtcatctgt > 1:1182514/1‑62 (MQ=255)ccTTTATCCGTCTGGTTGATGCTGAAAATCTGCGTGTTGGTCGCGGCTTTTACTtcatctgt > 1:2727839/1‑62 (MQ=255)ccTTTATCCGTCTGGTTGATGCTGAAAATCTGCGTGTTGGTCGCGGCTTTTACTtcatctgt > 1:2409100/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGt > 1:1058747/1‑39 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:2330028/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:677532/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:567028/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:2950912/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:2571529/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:2533071/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:223447/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:1892763/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:1673396/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:1487340/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:1330595/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtca > 1:1293150/1‑62 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtc > 1:2970772/1‑61 (MQ=255) tttATCCGTCTGGTTGATGCTGAAAATCTGCGTGCTGGTCGCGGCTTTTACTtcatccgtc > 1:3081581/1‑61 (MQ=255) tGCTGAAAATCTGCGTGTTGGTCGCGGCTTTTACTTCATCTGTCATCTGCCCGTTGATGaaa > 1:1420891/1‑62 (MQ=255) tgtTGGTCGCGGCTTTTACTTCATCTGTCATCTGCCCGTTGATGAAATCTTTCATCGAACGt < 1:1052102/62‑1 (MQ=255) tgtTGGTCGCGGCTTTTACTTCATCTGTCATCTGCCCGTTGATGAAATCTTTCATCGAACGt < 1:2036173/62‑1 (MQ=255) gtTGGGCGCGGCTTTTACTTCATCTGTCATCTGCCCGTTGATGAAATCTTTCATCGAACGt < 1:985394/61‑1 (MQ=255) ttGGTCGCGGCTTTTACTTCATCTGTCATCTGCCCGTTGATGAAATCTTTCATCGAACGttt < 1:2056889/62‑1 (MQ=255) ttGGTCGCGGCTTTTACTTCATCTGTCATCTGCCCGTTGATGAAATCTTTCATCGAACGttt < 1:2424442/62‑1 (MQ=255) ttGGTCGCGGCTTTTACTTCATCTGTCATCTGCCCGTTGATGAAATCTTTCATCGAACGttt < 1:777740/62‑1 (MQ=255) | CCTTTATCCGTCTGGTTGATGCTGAAAATCTGCGTGTTGGTCGCGGCTTTTACTTCATCTGTCATCTGCCCGTTGATGAAATCTTTCATCGAACGTTT > W3110S.gb/3492229‑3492326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |