| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,551,647 | G→A | T263T (ACG→ACA) | fdoG → | formate dehydrogenase‑O, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,551,647 | 0 | G | A | 92.3% | 71.5 / ‑1.1 | 26 | T263T (ACG→ACA) | fdoG | formate dehydrogenase‑O, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (24/0); total (24/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.08e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
AAAATTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACGCGTACGGCGGCGGTAGCTGACTACTATGCCCCTATTC > W3110S.gb/3551600‑3551684 | aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:2430782/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:995611/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:985805/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:800924/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:65958/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:343029/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:314111/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:29575/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:29145/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:2722585/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:2450539/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1073997/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:232437/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:212749/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:2121603/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1814735/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1623567/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1609282/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:157838/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:138046/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1358941/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1233678/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagcg > 1:1116483/1‑57 (MQ=39)aaaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACACGTAcagc > 1:1086437/1‑56 (MQ=39) aaaTTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACGCGTACGGCGGCGGTa < 1:3079755/62‑1 (MQ=255) gATTGTGATCGATCCTCGCTTTACGCGTACGGCGGCGGTAGCTGACTACTATGCCCCTATTc < 1:2734746/62‑1 (MQ=255) | AAAATTCACAACGGCGCGAAGCTGATTGTGATCGATCCTCGCTTTACGCGTACGGCGGCGGTAGCTGACTACTATGCCCCTATTC > W3110S.gb/3551600‑3551684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |