| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,660,197 | G→A | Y69Y (TAC→TAT) | wzxE ← | O‑antigen translocase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,660,197 | 0 | G | A | 83.8% | 83.8 / 10.1 | 37 | Y69Y (TAC→TAT) | wzxE | O‑antigen translocase |
| Reads supporting (aligned to +/- strand): ref base G (2/4); new base A (0/31); total (2/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
ACCAAGTACCATCGCTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATGGTACTGGGCAACGTATTTGGTTACACCGTTAAAGATGCCAGCCCCGGCAAGCA > W3110S.gb/3660137‑3660249 | aCCAAGTACCATCGCTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATGGt < 1:1115478/62‑1 (MQ=255)aCCAAGTACCATCGCTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATGGt < 1:2757703/62‑1 (MQ=255)aCCAAGTACCATCGCTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATGGt < 1:2427949/62‑1 (MQ=255)aCCAAGTACCATCGCTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATGGt < 1:2218724/62‑1 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:997850/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:881019/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:82967/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:751623/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:68975/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:6118/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:2837803/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:2164052/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:2160371/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:1837672/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:1502683/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:121680/1‑47 (MQ=255) cTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:1637444/1‑47 (MQ=255) gCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:2961274/1‑37 (MQ=255) gCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:269243/1‑37 (MQ=255) gCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:1399143/1‑37 (MQ=255) gCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:2406384/1‑37 (MQ=255) gCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:2282060/1‑37 (MQ=255) gCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:1560667/1‑37 (MQ=255) gCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:2818909/1‑37 (MQ=255) gCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:1143209/1‑37 (MQ=255) gCCGACCACGCGGCGCAGCTGTTGCGGATTATCATgg > 1:1908173/1‑37 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:484140/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:425422/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:3038254/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:746019/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:485767/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:49962/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:53251/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:58074/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:1462854/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:1018239/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:13203/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:781762/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:887472/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:895275/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:1679351/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:2879394/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:1754642/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:1667166/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:194889/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:2048984/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:2122203/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:1652899/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:1538401/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:2606908/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:2606913/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:2621660/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:2668881/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:2671901/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:14929/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:2854432/50‑2 (MQ=255) ggcgCAGCTGTTGGGGATTATCATGATACTGGGCAACGTATTTGGTTacg < 1:3018547/50‑2 (MQ=255) aTTATCATGGTACTGGGCAACGTATTTGGTTACACCGTTAAAGATGCCAGCCCCGGCAAGc > 1:204515/1‑61 (MQ=255) aTTATCATGGTACTGGGCAACGTATTTGGTTACACCGTTAAAGATGCCAGCCCCGGCAAGCa > 1:2508652/1‑62 (MQ=255) | ACCAAGTACCATCGCTGATGAAGTGCCGACCACGCGGCGCAGCTGTTGCGGATTATCATGGTACTGGGCAACGTATTTGGTTACACCGTTAAAGATGCCAGCCCCGGCAAGCA > W3110S.gb/3660137‑3660249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |