| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,663,442 | C→A | A240S (GCG→TCG) | rffG ← | dTDP‑glucose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,663,442 | 0 | C | A | 100.0% | 10.7 / NA | 5 | A240S (GCG→TCG) | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/5); total (0/5) | |||||||||||
CACCAATATTATAGGTTTCACCGACTTTCCCGGTGGTCGCCACGCAATACAGCGCGCGGGCG > W3110S.gb/3663403‑3663464 | caccaATATTATAGGTTTCACCGACTTTCCCGGTGGTCGACACGCAATACAGCGCGCGGGCg < 1:2220430/62‑1 (MQ=255)caccaATATTATAGGTTTCACCGACTTTCCCGGTGGTCGACACGCAATACAGCGCGCGGGCg < 1:2593552/62‑1 (MQ=255)caccaATATTATAGGTTTCACCGACTTTCCCGGTGGTCGACACGCAATACAGCGCGCGGGCg < 1:2943159/62‑1 (MQ=255) accaATATTATAGGTTTCACCGACTTTCCCGGTGGTCGACACGCAATACAGCGCGCGGGCg < 1:1570072/61‑1 (MQ=255) ccaATATTATAGGTTTCACCGACTTTCCCGGTGGTCGACACGCAATACAGCGCGCGGGCg < 1:418207/60‑1 (MQ=255) | CACCAATATTATAGGTTTCACCGACTTTCCCGGTGGTCGCCACGCAATACAGCGCGCGGGCG > W3110S.gb/3663403‑3663464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |