Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,702,210	C→A	S232S (TCG→TCT)	rbsA ←	fused D‑ribose transporter subunits and ATP‑binding components ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,702,210	0	C	A	100.0%	57.3 / NA	19	S232S (TCG→TCT)	rbsA	fused D‑ribose transporter subunits and ATP‑binding components ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/0);  total (19/0)

ATCTCAATCAGCGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGCCCATC  >  W3110S.gb/3702199‑3702260            |                                                   atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACtt                           >  1:1372477/1‑37 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGtccgt   >  1:48402/1‑59 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGtccgt   >  1:2270695/1‑59 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:830391/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:1088303/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:478932/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:3058716/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:2824951/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:274055/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:2719110/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:2435714/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:2348893/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:2073177/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:1598712/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:1592282/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:1523254/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:1335536/1‑62 (MQ=255) atcTCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:1157718/1‑62 (MQ=255) atcGCAATCAGAGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGTCCGTc  >  1:1281722/1‑62 (MQ=255)            |                                                   ATCTCAATCAGCGAATCTTCGGTCAGTGATGCCACTTCGCGCTCAGCAATAAATTGCCCATC  >  W3110S.gb/3702199‑3702260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑