Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	616,265	A→G	E962E (GAA→GAG)	entF →	enterobactin synthase multienzyme complex component, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	616,265	0	A	G	100.0%	127.2 / NA	36	E962E (GAA→GAG)	entF	enterobactin synthase multienzyme complex component, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/17);  total (19/17)

ACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTG  >  W3110S.gb/616214‑616308                                                    |                                            aCCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGcac                                   <  1:1598498/62‑1 (MQ=255)                 ggCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGcac                                   <  1:35594/46‑1 (MQ=255)                 ggCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGcac                                   <  1:1011871/46‑1 (MQ=255)                 ggCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGcac                                   <  1:2205543/46‑1 (MQ=255)                 ggCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGcac                                   <  1:2102610/46‑1 (MQ=255)                 ggCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGcac                                   <  1:43482/46‑1 (MQ=255)                 ggCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGcac                                   <  1:64123/46‑1 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAGGGCACAAGCGcc                            >  1:2140313/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAgagc                             >  1:3003800/1‑45 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:44365/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:389725/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:2613721/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:3112962/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:3067144/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:2926207/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:2712945/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:696781/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:799535/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:863843/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:184811/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:1256754/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:1608440/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:1585194/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:1427217/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:1373706/1‑46 (MQ=255)                        tGGATCGCAAAGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGcc                            >  1:1298673/1‑46 (MQ=255)                                  aGCCTTACCGTTGCCTTAGCTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTg  <  1:1407029/62‑1 (MQ=255)                                  aGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTg  <  1:1083466/62‑1 (MQ=255)                                  aGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTg  <  1:2019810/62‑1 (MQ=255)                                  aGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTg  <  1:822666/62‑1 (MQ=255)                                  aGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTg  <  1:764738/62‑1 (MQ=255)                                  aGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTg  <  1:716678/62‑1 (MQ=255)                                  aGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTg  <  1:1660228/62‑1 (MQ=255)                                  aGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTg  <  1:30518/62‑1 (MQ=255)                                  aGCCTTACCGTTGCCTGAGCTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTg  <  1:2797224/62‑1 (MQ=255)                                            ttGCCTGAGCTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTg  <  1:1732754/52‑1 (MQ=255)                                                 tgagctgaaGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTg  <  1:1875728/47‑1 (MQ=255)                                                    |                                            ACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTG  >  W3110S.gb/616214‑616308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑