Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,915,008	C→T	intergenic (+4/‑91)	ysaB → / → glyQ	hypothetical protein/glycine tRNA synthetase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,915,008	0	C	T	100.0%	30.5 / NA	11	intergenic (+4/‑91)	ysaB/glyQ	hypothetical protein/glycine tRNA synthetase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/9);  total (2/9)

TGTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCA  >  W3110S.gb/3914957‑3915045                                                    |                                      tGTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCTTGCTTTTTcc                             <  1:1702000/62‑1 (MQ=255)        ttGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCTTGCtttt                                >  1:1260111/1‑52 (MQ=255)                    gCCATTTTTTGCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCt          <  1:444148/62‑1 (MQ=255)                             tGCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTtccatcca  <  1:1039659/61‑1 (MQ=255)                             tGCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTtccatcca  <  1:1320166/61‑1 (MQ=255)                             tGCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTtccatcca  <  1:1777612/61‑1 (MQ=255)                             tGCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTtccatcca  <  1:468637/61‑1 (MQ=255)                             tGCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTtccatcca  <  1:534777/61‑1 (MQ=255)                             tGCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTtccatcca  <  1:882309/61‑1 (MQ=255)                             tGCATCTTTCCATGAGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTtccatcca  <  1:2708502/61‑1 (MQ=255)                                 tCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCg                      >  1:1183657/1‑37 (MQ=255)                                                    |                                      TGTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCA  >  W3110S.gb/3914957‑3915045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑