BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,931,020	C→T	A360V (GCG→GTG)	eptB →	predicted metal dependent hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,931,020	0	C	T	100.0%	145.5 / NA	47	A360V (GCG→GTG)	eptB	predicted metal dependent hydrolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/28); total (19/28)

AATGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGCGGAGCCACGTAATCGTGGCAA > W3110S.gb/3930961‑3931041

aaTGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTgg                     >  1:1184686/1‑62 (MQ=255)

aaTGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTgg                     >  1:42915/1‑62 (MQ=255)

aaTGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTgg                     >  1:2313029/1‑62 (MQ=255)

aaTGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTgg                     >  1:2214069/1‑62 (MQ=255)

aaTGTGGGTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTgg                     >  1:2812457/1‑62 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCa                >  1:1042460/1‑58 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCa                >  1:2195669/1‑58 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:2969886/1‑59 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:2980838/1‑59 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:3029260/1‑59 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:423437/1‑59 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:2130997/1‑59 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:757661/1‑59 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:1882634/1‑59 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:1037894/1‑59 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:1198829/1‑59 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:1820693/1‑59 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:1438143/1‑59 (MQ=255)

         cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc               >  1:834786/1‑59 (MQ=255)

                   acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa  <  1:1877062/62‑1 (MQ=255)

                   acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa  <  1:1164398/62‑1 (MQ=255)

                   acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa  <  1:2763416/62‑1 (MQ=255)

                   acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa  <  1:444205/62‑1 (MQ=255)

                   acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa  <  1:681778/62‑1 (MQ=255)

                   acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa  <  1:151054/62‑1 (MQ=255)

                   acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa  <  1:768822/62‑1 (MQ=255)

                   acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa  <  1:2047099/62‑1 (MQ=255)

                   acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa  <  1:755746/62‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:400951/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:779415/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:3078698/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:863453/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:2828256/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:282609/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:2806360/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:2776734/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:2729455/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:2627034/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:2607760/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:2588607/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:1926922/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:192269/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:1850510/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:175031/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:1471500/39‑1 (MQ=255)

                                     gCTTATCGTGAGCAGATTGGTGTGAAGCCACGTAATCGt       <  1:2459647/39‑1 (MQ=38)

                                         aTCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt       <  1:1115013/35‑1 (MQ=255)

AATGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGCGGAGCCACGTAATCGTGGCAA > W3110S.gb/3930961‑3931041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`