Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,050,892	C→T	T168M (ACG→ATG)	ugpE →	glycerol‑3‑phosphate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,050,892	0	C	T	100.0%	53.2 / NA	18	T168M (ACG→ATG)	ugpE	glycerol‑3‑phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/14);  total (4/14)

GCTGCCGCTGATGGCCTCGGCGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGACGCTGCCGGATGAGCTGGTGGAAGCCGCGCGGATCGACGGCGCATCGCCAAT  >  W3110S.gb/4050836‑4050943                                                         |                                                    gCTGCCGCTGATGGGCTCGGCGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTg                                                 <  1:1187035/61‑1 (MQ=255) gCTGCCGCTGATGGCCTCGGCGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTg                                                 <  1:1332776/61‑1 (MQ=255) gCTGCCGCTGATGGCCTCGGCGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTg                                                 <  1:2926338/61‑1 (MQ=255) gCTGCCGCTGATGGCCTCGGCGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTg                                                 <  1:1129137/61‑1 (MQ=255) gCTGCCGCTGATGGCCTCGGCGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTg                                                 <  1:2776441/61‑1 (MQ=255)    gCCGCTGATGGCCTCGGCGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTGCCgg                                             >  1:3127530/1‑62 (MQ=255)                     cGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTGCCGGATGAGCTGGTGGAAGcc                            <  1:2576269/62‑1 (MQ=255)                     cGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTGCCGGATGAGCTGGTGGAAGcc                            <  1:847023/62‑1 (MQ=255)                     cGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTGCCGGATGAGCTGGTGGAAGcc                            <  1:659803/62‑1 (MQ=255)                     cGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTGCCGGATGAGCTGGTGGAAGcc                            <  1:296548/62‑1 (MQ=255)                     cGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTGCCGGATGAGCTGGTGGAAGcc                            <  1:2862965/62‑1 (MQ=255)                     cGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTGCCGGATGAGCTGGTGGAAGcc                            <  1:2170816/62‑1 (MQ=255)                     cGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTGCCGGATGAGCTGGTGGAAGcc                            <  1:1280255/62‑1 (MQ=255)                     cGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTGCCGGATGAGCTGGTGGAAGcc                            <  1:1226337/62‑1 (MQ=255)                     cGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGATGCTGCCGGATGAGCTGGTGGAAGcc                            <  1:116119/62‑1 (MQ=255)                                               ttCTTTATGATGCTGCCGGATGAGCTGGTGGAAGCCGCGCGGATCGACGGCGCATCGCCAAt  >  1:2249158/1‑62 (MQ=255)                                               ttCTTTATGATGCTGCCGGATGAGCTGGTGGAAGCCGCGCGGATCGACGGCGCATCGCCAAt  >  1:2225597/1‑62 (MQ=255)                                               ttCTTTATGATGCTGCCGGATGAGCTGGTGGAAGCCGCGCGGATCGACGGCGCATCGCCAAt  >  1:472804/1‑62 (MQ=255)                                                         |                                                    GCTGCCGCTGATGGCCTCGGCGACCGCTACTTTCCTGTTCCGCCAGTTCTTTATGACGCTGCCGGATGAGCTGGTGGAAGCCGCGCGGATCGACGGCGCATCGCCAAT  >  W3110S.gb/4050836‑4050943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑