| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,149,789 | C→T | R67R (CGC→CGT) | pabA → | aminodeoxychorismate synthase, subunit II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,149,789 | 0 | C | T | 75.0% | 58.3 / 19.1 | 36 | R67R (CGC→CGT) | pabA | aminodeoxychorismate synthase, subunit II |
| Reads supporting (aligned to +/- strand): ref base C (4/5); new base T (27/0); total (31/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.34e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCTGTACGCCAGATGAAGCCGGGATCTCCCTTGACGTTATTCGCCACTATGCCGGGCGCTTGCCGATTCTTGGCGTCTGCCTCGGTCATCAGGCAATGG > W3110S.gb/4149745‑4149844 | cccTGTACGCCAGATGAAGCCGGGATCTCCCTTGACGTTATTCGCCACTATGCCGGGCGCt < 1:118974/61‑1 (MQ=255)cccTGTACGCCAGATGAAGCCGGGATCTCCCTTGACGTTATTCGCCACTATGCCGGGCGCt < 1:2373792/61‑1 (MQ=255)cccTGTACGCCAGATGAAGCCGGGATCTCCCTTGACGTTATTCGCCACTATGCCGGGCGCt < 1:2351829/61‑1 (MQ=255)cccTGTACGCCAGATGAAGCCGGGATCTCCCTTGACGTTATTCGCCACTATGCCGGGCGCt < 1:1936957/61‑1 (MQ=255)cccTGTACGCCAGATGAAGCCGGGAGCTCCCTTGACGTTATTCGCCACTATGCCGGGCGCt < 1:378607/61‑1 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:2957537/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:99656/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:97524/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:857388/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:816134/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:799422/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:589476/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:532309/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:474309/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:383718/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:3089252/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:3042071/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:3014242/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:3006425/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:1300453/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:291619/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:1011940/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:124184/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:2305925/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:2253846/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:2117857/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:2082107/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:2017329/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:1989992/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:1254735/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:1797704/1‑45 (MQ=255) ctcCCTTGACGTTATTCGTCACTATGCCGGACGCTTGCCGATTCt > 1:1358862/1‑45 (MQ=255) tATTCGCCACTATGCCGGGCGCTTGCCGATTCTTGGCGTCTGCCTCGGTCATCAGGc > 1:174207/1‑57 (MQ=255) tATTCGCCACTATGCCGGGCGCTTGCCGATTCTTGGCGTCTGCCTCGGTCATCAGGc > 1:1404835/1‑57 (MQ=255) tATTCGCCACTATGCCGGGCGCTTGCCGATTCTTGGCGTCTGCCTCGGTCATCAGGCAATgg > 1:932434/1‑62 (MQ=255) tATTCGCCACTATGCCGGGCGCTTGCCGATTCTTGGCGTCTGCCTCGGTCATCAAGc > 1:2006456/1‑57 (MQ=255) | CCCTGTACGCCAGATGAAGCCGGGATCTCCCTTGACGTTATTCGCCACTATGCCGGGCGCTTGCCGATTCTTGGCGTCTGCCTCGGTCATCAGGCAATGG > W3110S.gb/4149745‑4149844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |