Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	692,265	G→A	intergenic (‑59/+31)	ybeX ← / ← ybeY	predicteed ion transport/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	692,265	0	G	A	100.0%	178.3 / NA	55	intergenic (‑59/+31)	ybeX/ybeY	predicteed ion transport/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/27);  total (28/27)

TCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTC  >  W3110S.gb/692211‑692305                                                       |                                         tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGAc                                     >  1:1740900/1‑60 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACg                                    >  1:1200445/1‑61 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGt                                   >  1:830327/1‑62 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGt                                   >  1:565284/1‑62 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGt                                   >  1:3033786/1‑62 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGt                                   >  1:3030030/1‑62 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGt                                   >  1:29626/1‑62 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGt                                   >  1:2584570/1‑62 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGt                                   >  1:1983406/1‑62 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGt                                   >  1:1353342/1‑62 (MQ=255)     aTGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:1080104/61‑1 (MQ=255)     aTGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:2975748/61‑1 (MQ=255)     aTGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:1899264/61‑1 (MQ=255)     aTGGGTGCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:130742/61‑1 (MQ=255)          ttCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGAc                                     >  1:2192991/1‑51 (MQ=255)          ttCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGt                           >  1:2881286/1‑61 (MQ=255)          ttCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGt                           >  1:1096532/1‑61 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAAGGGCAGGGACGTCTg                                <  1:2448685/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:137107/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:1213296/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:1309626/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:1175182/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:1764226/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:1664464/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:549214/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:1595207/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:601698/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:148803/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:719945/48‑1 (MQ=255)                  ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCAGGGACGTCTg                                <  1:1379810/48‑1 (MQ=255)                               aCGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:1939263/1‑42 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGTGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt    <  1:1344470/62‑1 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:444732/1‑41 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:1059086/1‑41 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:2522592/1‑41 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:2635078/1‑41 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:2631565/1‑41 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:2578970/1‑41 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:2561684/1‑41 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:2427674/1‑41 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:2251711/1‑41 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:1275542/1‑41 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCa                         >  1:1414767/1‑41 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt    <  1:1182849/62‑1 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt    <  1:1170119/62‑1 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt    <  1:427528/62‑1 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt    <  1:53500/62‑1 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt    <  1:1609213/62‑1 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt    <  1:1582053/62‑1 (MQ=255)                                cGCCGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt    <  1:1532390/62‑1 (MQ=255)                                  ccGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCTGGCAAatt          >  1:1894118/1‑54 (MQ=255)                                  ccGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctttc  >  1:457089/1‑62 (MQ=255)                                  ccGTCGTTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt    >  1:133471/1‑60 (MQ=255)                                       gtTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCt                  <  1:1454643/41‑1 (MQ=255)                                       gtTAATCACCAACGGCAGGGACGTCTGCCAGTCAAATGCCt                  <  1:966261/41‑1 (MQ=255)                                                       |                                         TCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTC  >  W3110S.gb/692211‑692305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑