Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,302,518	A→G	R110R (CGT→CGC)	fdhF ←	formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,302,518	0	A	G	80.0%	14.6 / ‑0.5	10	R110R (CGT→CGC)	fdhF	formate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (0/8);  total (1/9)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

CGCAAATTTTTGCATTACATAGTTGGTTTCGTTACCCGTACCACGCGAGGAGCCGGTCGTCTGGA  >  W3110S.gb/4302476‑4302540                                           |                       cgcAAATTTTTGCATTACATAGTTGGTTTCGTTACCCGTACCACGCGAGGAGCCGGTCGTCt     <  1:1226355/62‑1 (MQ=255)                tACATAGTTGGTTTCGTTACCCGTACCACGCGAGGAGCCGGTCGTCTGGa  >  1:356086/1‑50 (MQ=255)                   aTAGTTGGTTTCGTTACCCGTACCGCGCGAGGAGCCGGTCGTCTGGa  <  1:1589162/47‑1 (MQ=255)                   aTAGTTGGTTTCGTTACCCGTACCGCGCGAGGAGCCGGTCGTCTGGa  <  1:2612313/47‑1 (MQ=255)                   aTAGTTGGTTTCGTTACCCGTACCGCGCGAGGAGCCGGTCGTCTGGa  <  1:2946410/47‑1 (MQ=255)                   aTAGTTGGTTTCGTTACCCGTACCGCGCGAGGAGCCGGTCGTCTGGa  <  1:2952312/47‑1 (MQ=255)                   aTAGTTGGTTTCGTTACCCGTACCGCGCGAGGAGCCGGTCGTCTGGa  <  1:385429/47‑1 (MQ=255)                   aTAGTTGGTTTCGTTACCCGTACCGCGCGAGGAGCCGGTCGTCTGGa  <  1:819568/47‑1 (MQ=255)                       ttGGTTTCGTTACCCGTACCGCGCGAGGAGCCGGTCGTCTGGa  <  1:1978482/43‑1 (MQ=255)                             tCGTTACCCGTACCGCGCGAGGAGCCGGTCGTCTGGa  <  1:2652336/37‑1 (MQ=255)                                           |                       CGCAAATTTTTGCATTACATAGTTGGTTTCGTTACCCGTACCACGCGAGGAGCCGGTCGTCTGGA  >  W3110S.gb/4302476‑4302540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑