Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,431,194	C→T	H135H (CAC→CAT)	rplI →	50S ribosomal subunit protein L9

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,431,194	0	C	T	100.0%	68.5 / NA	22	H135H (CAC→CAT)	rplI	50S ribosomal subunit protein L9
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/3);  total (19/3)

GCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTC  >  W3110S.gb/4431148‑4431249                                               |                                                        gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGc                                                      >  1:875886/1‑50 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:342100/1‑51 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:328150/1‑51 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:323989/1‑51 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:575332/1‑51 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:592711/1‑51 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:2822168/1‑51 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:2696634/1‑51 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:623174/1‑51 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:2530830/1‑51 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:2117455/1‑51 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:1940366/1‑51 (MQ=255) gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg                                                     >  1:702460/1‑51 (MQ=255)                       acacGAAGTGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGtagtag                    <  1:2784635/62‑1 (MQ=255)                       acacGAAGTGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGtagtag                    <  1:102492/62‑1 (MQ=255)                       acacGAAGTGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGtagtag                    <  1:237550/62‑1 (MQ=255)                               tGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGaataa            >  1:282465/1‑62 (MQ=255)                               tGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGaataa            >  1:2942774/1‑62 (MQ=255)                               tGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGaataa            >  1:2916137/1‑62 (MQ=255)                               tGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGaataa            >  1:2675999/1‑62 (MQ=255)                               tGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGaata             >  1:1514140/1‑61 (MQ=255)                                         ggTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTc  >  1:1264321/1‑62 (MQ=255)                                               |                                                        GCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTC  >  W3110S.gb/4431148‑4431249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑