Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,457,723	G→C	G156G (GGG→GGC)	ytfT →	predicted sugar transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,457,723	0	G	C	92.9%	39.5 / ‑3.3	14	G156G (GGG→GGC)	ytfT	predicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (0/13);  total (0/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATG  >  W3110S.gb/4457684‑4457758                                        |                                    ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:1199813/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:1365100/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:1587618/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:1718100/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:20525/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:2129718/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:2143036/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:2655365/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:2708432/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:2708737/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:2895941/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCTGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:839165/51‑1 (MQ=255) ggTGGCGGGCCGCGGCCGGGCGCAGTTGCTGACCGACGGCCAGATCGTCAc                          <  1:772625/51‑1 (MQ=255)               gcgTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATg  <  1:2390815/61‑1 (MQ=255)                                        |                                    GGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATG  >  W3110S.gb/4457684‑4457758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑