Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,563,589	T→A	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,563,589	0	T	A	100.0%	87.7 / NA	29	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (29/0);  total (29/0)

CAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCG  >  W3110S.gb/4563564‑4563636                          |                                                cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg             >  1:86054/1‑62 (MQ=255) cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg             >  1:1023715/1‑62 (MQ=255) cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg             >  1:2763051/1‑62 (MQ=255)             aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg  >  1:2866086/1‑61 (MQ=255)             aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg  >  1:883596/1‑61 (MQ=255)             aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg  >  1:724893/1‑61 (MQ=255)             aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg  >  1:531968/1‑61 (MQ=255)             aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg  >  1:2088842/1‑61 (MQ=255)             aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg  >  1:2038835/1‑61 (MQ=255)             aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg  >  1:1570301/1‑61 (MQ=255)             aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg  >  1:1165919/1‑61 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                      >  1:564017/1‑37 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:1094315/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:1915396/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:110492/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:790763/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:756970/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:745374/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:639250/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:624021/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:579731/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:1437500/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:377593/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:3053138/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:2899186/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:1519205/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:258823/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:2203953/1‑38 (MQ=255)                 tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                     >  1:1698142/1‑38 (MQ=255)                          |                                                CAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCG  >  W3110S.gb/4563564‑4563636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑