Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	726,557	A→G	V301A (GTT→GCT)	kdpB ←	potassium translocating ATPase, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	726,557	0	A	G	100.0%	30.9 / NA	10	V301A (GTT→GCT)	kdpB	potassium translocating ATPase, subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/9);  total (1/9)

TGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACG  >  W3110S.gb/726520‑726582                                      |                          tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc   <  1:1002254/62‑1 (MQ=255) tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc   <  1:1108801/62‑1 (MQ=255) tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc   <  1:1866395/62‑1 (MQ=255) tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc   <  1:2507980/62‑1 (MQ=255) tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc   <  1:2926589/62‑1 (MQ=255) tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc   <  1:2935633/62‑1 (MQ=255) tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc   <  1:2972124/62‑1 (MQ=255) tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc   <  1:810767/62‑1 (MQ=255)  gtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCAc   <  1:251559/61‑1 (MQ=255)  gtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAGCGTCACCTGCCGCTTCAACTGCACg  >  1:247074/1‑62 (MQ=255)                                      |                          TGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACG  >  W3110S.gb/726520‑726582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑