| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 776,135 | G→C | P187P (CCG→CCC) | tolQ → | membrane spanning protein in TolA‑TolQ‑TolR complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 776,135 | 0 | G | C | 100.0% | 12.2 / NA | 5 | P187P (CCG→CCC) | tolQ | membrane spanning protein in TolA‑TolQ‑TolR complex |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (5/0); total (5/0) | |||||||||||
CCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGT > W3110S.gb/776080‑776140 | cgggTATTGCCGAAGCCCTGATCGCCACCGCGATCGGCCTGTTCGCCGCTATCCCCGCAGt > 1:2309689/3‑61 (MQ=255)cgggTATTGCCGAAGCCCTGATCGCCACCGCGATCGGCCTGTTCGCCGCTATCCCCGCAGt > 1:2825727/3‑61 (MQ=255)cgggTATTGCCGAAGCCCTGATCGCCACCGCGATCGGCCTGTTCGCCGCTATCCCCGCAGt > 1:2884175/3‑61 (MQ=255)cgggTATTGCCGAAGCCCTGATCGCCACCGCGATCGGCCTGTTCGCCGCTATCCCCGCAGt > 1:437743/3‑61 (MQ=255)cgggTATTGCCGAAGCCCTGATCGCCACCGAGATCGGCCTGTTCGCCGCTATCCCCGCAGt > 1:1781066/3‑61 (MQ=255) | CCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGT > W3110S.gb/776080‑776140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |