Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	836,257	G→T	A196A (GCG→GCT)	ybiB →	predicted transferase/phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	836,257	0	G	T	100.0%	34.7 / NA	12	A196A (GCG→GCT)	ybiB	predicted transferase/phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/4);  total (8/4)

GCACATACCCTGGCGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCG  >  W3110S.gb/836210‑836303                                                |                                               gCACATACCCTGGCGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCTCTGCGTCTTTCCAg                                  >  1:140337/1‑62 (MQ=255) gCACATACCCTGGCGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCTCTGCGTCTTTCCAg                                  >  1:1448827/1‑62 (MQ=255) gCACATACCCTGGCGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCTCTGCGTCTTTCCAg                                  >  1:2068506/1‑62 (MQ=255) gCACATACCCTGGCGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCTCTGCGTCTTTCCAg                                  >  1:2131307/1‑62 (MQ=255) gCACATACCCTGGCGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCTCTGCGTCTTTCCAg                                  >  1:347195/1‑62 (MQ=255) gCACATACCCTGGCGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCTCTGCGTCTTTCCAg                                  >  1:548942/1‑62 (MQ=255)     aTACACTGGCGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCTCTGCGTCTTTCCAGCGtt                              >  1:503659/1‑62 (MQ=255)                        gACGCCATTTGCCGAAGGTGAGGCTCTGCGTGTTTCCAGGGTTTCGCATCCg                     <  1:3011412/52‑1 (MQ=255)                        gACGCCATTTGCCGAAGGTGAGGCTCTGCGTCTTTCCAGCGTTTCGCATCCg                     <  1:2087610/52‑1 (MQ=255)                        gACGCCATTTGCCGAAGGTGAGGCTCTGCGTCTTTCCAGCGTTTCGCATCCg                     <  1:827128/52‑1 (MQ=255)                                  gCCGAAGGTGAGGCTCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTcg  >  1:1056208/1‑61 (MQ=255)                                         gTGAGGCTCTGCGTCTTTCCAGCGTTTCGCATCCg                     <  1:1745614/35‑1 (MQ=255)                                                |                                               GCACATACCCTGGCGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCG  >  W3110S.gb/836210‑836303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑