Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	154,121	C→T	D436N (GAT→AAT)	htrE ←	predicted outer membrane usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	154,121	0	C	T	100.0%	63.4 / NA	21	D436N (GAT→AAT)	htrE	predicted outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/13);  total (8/13)

TTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACAT  >  W3110S.gb/154064‑154153                                                          |                                 tttCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATcc                              >  1:1072400/1‑62 (MQ=255) tttCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATcc                              >  1:628667/1‑62 (MQ=255) tttCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATcc                              >  1:3080285/1‑62 (MQ=255) tttCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATcc                              >  1:1514835/1‑62 (MQ=255) tttCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATcc                              >  1:1516709/1‑62 (MQ=255)         aaTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATa                        <  1:995708/60‑1 (MQ=255)         aaTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATa                        <  1:1065989/60‑1 (MQ=255)         aaTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATa                        <  1:879343/60‑1 (MQ=255)          aTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATACGa                     <  1:1803553/62‑1 (MQ=255)          aTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATACGa                     <  1:139727/62‑1 (MQ=255)          aTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATACGa                     <  1:427899/62‑1 (MQ=255)          aTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATACGa                     <  1:539375/62‑1 (MQ=255)          aTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATACGa                     <  1:1324897/62‑1 (MQ=255)          aTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATACGa                     <  1:697251/62‑1 (MQ=255)          aTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATACGa                     <  1:1320017/62‑1 (MQ=255)            aaCTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATa                        <  1:1258629/57‑1 (MQ=255)                   tCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATACGAACATTgg              <  1:2064874/60‑1 (MQ=255)                    ccAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATACGAACATTGGa             >  1:2529508/1‑60 (MQ=255)                    ccAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATACGAACATTGGa             >  1:925717/1‑60 (MQ=255)                      aGGAAACACGATAACTTTGCCCCTGGTATGTTTTATTATCCGGGATACGAACATTGGAATGa         >  1:829058/1‑62 (MQ=255)                             acGATAACTTTGCCCCAGGTATGTTTTATTATCCGGGATACGAACATTGGAATGAGTCACAt  <  1:74977/62‑1 (MQ=255)                                                          |                                 TTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACAT  >  W3110S.gb/154064‑154153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑