Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	875,632	C→T	S744S (AGC→AGT)	yliE →	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	875,632	0	C	T	97.9%	145.2 / ‑6.2	47	S744S (AGC→AGT)	yliE	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/30);  minor base G (0/1);  total (16/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAG  >  W3110S.gb/875595‑875680                                      |                                                 ataACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATc                          <  1:338684/62‑1 (MQ=255) ataACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATc                          <  1:2713828/62‑1 (MQ=255) ataACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATc                          <  1:2486902/62‑1 (MQ=255) ataACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATc                          <  1:2483433/62‑1 (MQ=255) ataACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATc                          <  1:2445116/62‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:9716/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:513/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:2685755/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:2270027/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:2088057/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:2045111/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:1987663/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:898791/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:157639/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:173656/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:1784017/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:1819293/44‑1 (MQ=255)    aCCGGATCCCGGTGATTGCAGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:1901275/44‑1 (MQ=38)          tCCCGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGtt                                         <  1:1302196/38‑1 (MQ=255)           cccGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGa                 >  1:2744062/1‑61 (MQ=255)           cccGGTGATTGCGGAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGAt                >  1:2321942/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGCCGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:1174878/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGCCGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:2481236/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTa                             >  1:660917/1‑37 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:3008647/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:304225/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:2770675/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:962529/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:1919295/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:1194270/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:2428895/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:2381594/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:2153916/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:2015715/1‑62 (MQ=255)                       ggAAGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTc    >  1:745748/1‑62 (MQ=255)                         aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:647389/62‑1 (MQ=255)                         aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:464973/62‑1 (MQ=255)                         aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:992518/62‑1 (MQ=255)                         aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:3089739/62‑1 (MQ=255)                         aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:3013224/62‑1 (MQ=255)                         aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:2367505/62‑1 (MQ=255)                         aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:1936723/62‑1 (MQ=255)                         aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:1867837/62‑1 (MQ=255)                         aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:1181957/62‑1 (MQ=255)                         aaGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:1024827/62‑1 (MQ=255)                          aGGCGTAGAGAGTGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:1503529/61‑1 (MQ=255)                          aGGCGTAGAGAGGGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAg  <  1:1015200/61‑1 (MQ=255)                                      |                                                 ATAACCGGATCCCGGTGATTGCGGAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAG  >  W3110S.gb/875595‑875680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑