Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	916,990	G→A	A32A (GCG→GCA)	ybjD →	conserved hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	916,990	0	G	A	100.0%	34.2 / NA	12	A32A (GCG→GCA)	ybjD	conserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/5);  total (7/5)

CCGTTTGTCGTTGATGCTGGAACAAAACAACGTCCTGATTGGGGAGAACGCGTGGGGTAAATCCAGCTTGCTGGACGCCTTAACTCTGCTGCTATCGCCAGAATCAGA  >  W3110S.gb/916939‑917046                                                    |                                                         ccGTTTGTCGTTGATGCTGGAACAAAACAACGTCCTGATTGGGGAGAACGCATGGGGTAAAt                                                <  1:1286969/62‑1 (MQ=255) ccGTTTGTCGTTGATGCTGGAACAAAACAACGTCCTGATTGGGGAGAACGCATGGGGTAAAt                                                <  1:1842492/62‑1 (MQ=255) ccGTTTGTCGTTGATGCTGGAACAAAACAACGTCCTGATTGGGGAGAACGCATGGGGTAAAt                                                <  1:2548845/62‑1 (MQ=255) ccGTTTGTCGTTGATGCTGGAACAAAACAACGTCCTGATTGGGGAGAACGCATGGGGTAAAt                                                <  1:2671404/62‑1 (MQ=255) ccGTTTGTCGTTGATGCTGGAACAAAACAACGTCCTGATTGGGGAGAACGCATGGGGTAAAt                                                <  1:284219/62‑1 (MQ=255)             gATGCTGGAACAAAACAACGTCCTGATTGGGGAGAACGCATGGGGTAAATCCAGCTTGCTg                                     >  1:2688200/1‑61 (MQ=255)                                               aaCGCATGGGGTAAATCCAGCTTGCTGGACGCCTTAACTCTGCTGCTATCGCCAGAATCAGa  >  1:1408830/1‑62 (MQ=255)                                               aaCGCATGGGGTAAATCCAGCTTGCTGGACGCCTTAACTCTGCTGCTATCGCCAGAATCAGa  >  1:1753954/1‑62 (MQ=255)                                               aaCGCATGGGGTAAATCCAGCTTGCTGGACGCCTTAACTCTGCTGCTATCGCCAGAATCAGa  >  1:2436142/1‑62 (MQ=255)                                               aaCGCATGGGGTAAATCCAGCTTGCTGGACGCCTTAACTCTGCTGCTATCGCCAGAATCAGa  >  1:2582697/1‑62 (MQ=255)                                               aaCGCATGGGGTAAATCCAGCTTGCTGGACGCCTTAACTCTGCTGCTATCGCCAGAATCAGa  >  1:2822778/1‑62 (MQ=255)                                               aaCGCATGGGGTAAATCCAGCTTGCTGGACGCCTTAACTCTGCTGCTATCGCCAGAATCAGa  >  1:783438/1‑62 (MQ=255)                                                    |                                                         CCGTTTGTCGTTGATGCTGGAACAAAACAACGTCCTGATTGGGGAGAACGCGTGGGGTAAATCCAGCTTGCTGGACGCCTTAACTCTGCTGCTATCGCCAGAATCAGA  >  W3110S.gb/916939‑917046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑