| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 919,992 | T→C | R112R (CGT→CGC) | macA → | macrolide transporter subunit, membrane fusion protein (MFP) component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 919,992 | 0 | T | C | 76.7% | 47.4 / 13.3 | 30 | R112R (CGT→CGC) | macA | macrolide transporter subunit, membrane fusion protein (MFP) component |
| Reads supporting (aligned to +/- strand): ref base T (6/1); new base C (23/0); total (29/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01 | |||||||||||
GAACAGGCTGAAAACCAGATTAAGGAGGTCGAAGCAACGCTGATGGAGCTACGTGCGCAGCGGCAGCAGGCGGAAGCGGAGCTGAAACTGGCGCGGGTGACGTATTCCCG > W3110S.gb/919939‑920048 | gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCGACGCTGATGGAGCTACGCGCGCAGc > 1:1609274/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:2518671/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:647532/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:638803/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:609018/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:576238/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:458772/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:404176/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:342247/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:3101010/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:3059424/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:2712275/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:2643698/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:2524692/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:2432587/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:2248772/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:1956319/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:1857398/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:1563269/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:1429780/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:1114313/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTGGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:1023846/1‑61 (MQ=255)gAACAGGCTGAAAACCAGATCAAGGAGGTCGAAGCAACGCTGATGGAGCTACGCGCGCAGc > 1:1269010/1‑61 (MQ=255) ggCTGAAAACCAGATTAAGGAGGTCGAAGCAACGCTGATGGAGCTACGTGCGCAGCGgcagc > 1:10072/1‑62 (MQ=255) ggCTGAAAACCAGATTAAGGAGGTCGAAGCAACGCTGATGGAGCTACGTGCGCAGCGgcagc > 1:2008633/1‑62 (MQ=255) ggCTGAAAACCAGATTAAGGAGGTCGAAGCAACGCTGATGGAGCTACGTGCGCAGCGgcagc > 1:2715544/1‑62 (MQ=255) ggCTGAAAACCAGATTAAGGAGGTCGAAGCAACGCTGATGGAGCTACGTGCGCAGCGgcagc > 1:30667/1‑62 (MQ=255) ggCTGAAAACCAGATTAAGGAGGTCGAAGCAACGCTGATGGAGCTACGTGCGCAGCGgcagc > 1:1264224/1‑62 (MQ=255) ggCTGAAAACCAGATTAAGGAGGTCGAAGCAACGCTGATGGAGCTACGTGCGCAGCGgcag > 1:23566/1‑61 (MQ=255) cTACGTGCGCAGCGGCAGCAGGCGGAAGCGGAGCTGAAACTGGCGCGGGTGACGTATTCCCg < 1:1182621/62‑1 (MQ=255) | GAACAGGCTGAAAACCAGATTAAGGAGGTCGAAGCAACGCTGATGGAGCTACGTGCGCAGCGGCAGCAGGCGGAAGCGGAGCTGAAACTGGCGCGGGTGACGTATTCCCG > W3110S.gb/919939‑920048 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |